Document Detail


Lunasin, with an arginine-glycine-aspartic acid motif, causes apoptosis to L1210 leukemia cells by activation of caspase-3.
MedLine Citation:
PMID:  19937853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lunasin is a novel chemopreventive peptide featuring a cell adhesion motif composed of arginine-glycine-aspartate (RGD) which has been associated to cytotoxicity to established cell lines. The objectives of this study were to determine the effect of lunasin on the viability of L1210 leukemia cells and to understand the underlying mechanisms involved. Pure lunasin and lunasin enriched soy flour (LES) caused cytotoxicity to L1210 leukemia cells with IC(50) of 14 and 16 microM (lunasin equivalent), respectively. Simulated gastrointestinal digestion showed that 25% of the original amount of lunasin survived 3 h of pepsin digestion and 3% of lunasin remained after sequential pepsin-pancreatin digestion for a total of 6 h. Cell cycle analysis showed that lunasin caused a dose-dependent G2 cell cycle arrest and apoptosis. Treatment of L1210 leukemia cells with 1 mg/mL of LES for 18 h led to an increase in the amount of apoptotic cells from 2 to 40%. Compared to untreated cells, treatment with 1 mg/mL LES showed a 6-fold increase on the expressions of caspases-8 and -9, and and a 12-fold increase on the expression of caspase-3. These results showed for the first time that lunasin, a naturally occurring peptide containing an RGD motif, caused apoptosis to L1210 leukemia cells through caspase-3 activation.
Authors:
Elvira Gonzalez de Mejia; Wenyi Wang; Vermont P Dia
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  406-14     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL 61801, USA. edemejia@illinois.edu
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs
Animals
Antineoplastic Agents, Phytogenic / chemistry,  metabolism,  pharmacology*
Apoptosis / drug effects*
Caspase 3 / metabolism*
Caspase 8 / metabolism
Caspase 9 / metabolism
Cell Line, Tumor
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Stability
Enzyme Activation
G2 Phase / drug effects
Hydrolases / metabolism
Inhibitory Concentration 50
Leukemia L1210 / drug therapy*,  metabolism,  pathology
Mice
Oligopeptides*
Phytotherapy
Plant Extracts / chemistry,  isolation & purification,  pharmacology
Soybean Proteins / chemistry,  metabolism,  pharmacology*
Time Factors
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/GM2S-1 protein, Glycine max; 0/Oligopeptides; 0/Plant Extracts; 0/Soybean Proteins; 99896-85-2/arginyl-glycyl-aspartic acid; EC 3.-/Hydrolases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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