| Lunasin, with an arginine-glycine-aspartic acid motif, causes apoptosis to L1210 leukemia cells by activation of caspase-3. | |
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MedLine Citation:
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PMID: 19937853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lunasin is a novel chemopreventive peptide featuring a cell adhesion motif composed of arginine-glycine-aspartate (RGD) which has been associated to cytotoxicity to established cell lines. The objectives of this study were to determine the effect of lunasin on the viability of L1210 leukemia cells and to understand the underlying mechanisms involved. Pure lunasin and lunasin enriched soy flour (LES) caused cytotoxicity to L1210 leukemia cells with IC(50) of 14 and 16 microM (lunasin equivalent), respectively. Simulated gastrointestinal digestion showed that 25% of the original amount of lunasin survived 3 h of pepsin digestion and 3% of lunasin remained after sequential pepsin-pancreatin digestion for a total of 6 h. Cell cycle analysis showed that lunasin caused a dose-dependent G2 cell cycle arrest and apoptosis. Treatment of L1210 leukemia cells with 1 mg/mL of LES for 18 h led to an increase in the amount of apoptotic cells from 2 to 40%. Compared to untreated cells, treatment with 1 mg/mL LES showed a 6-fold increase on the expressions of caspases-8 and -9, and and a 12-fold increase on the expression of caspase-3. These results showed for the first time that lunasin, a naturally occurring peptide containing an RGD motif, caused apoptosis to L1210 leukemia cells through caspase-3 activation. |
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Authors:
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Elvira Gonzalez de Mejia; Wenyi Wang; Vermont P Dia |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-07-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: 406-14 Citation Subset: IM |
Affiliation:
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Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL 61801, USA. edemejia@illinois.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Animals Antineoplastic Agents, Phytogenic / chemistry, metabolism, pharmacology* Apoptosis / drug effects* Caspase 3 / metabolism* Caspase 8 / metabolism Caspase 9 / metabolism Cell Line, Tumor Cell Survival / drug effects Dose-Response Relationship, Drug Drug Stability Enzyme Activation G2 Phase / drug effects Hydrolases / metabolism Inhibitory Concentration 50 Leukemia L1210 / drug therapy*, metabolism, pathology Mice Oligopeptides* Phytotherapy Plant Extracts / chemistry, isolation & purification, pharmacology Soybean Proteins / chemistry, metabolism, pharmacology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/GM2S-1 protein, Glycine max; 0/Oligopeptides; 0/Plant Extracts; 0/Soybean Proteins; 99896-85-2/arginyl-glycyl-aspartic acid; EC 3.-/Hydrolases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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