Document Detail


Luminol-dependent chemiluminescence microassay for phagocytic function.
MedLine Citation:
PMID:  3397547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A luminol-dependent chemiluminescence (CL) microassay was developed to measure phagocytic function of peripheral blood leukocytes. Buffy coats, obtained by centrifugation of only 100 microliter of whole blood, provided an enriched population of polymorphonuclear leukocytes (PMNs). The total reaction mixture, consisting of leukocytes-luminol-inducer (opsonized zymosan), was 450 microliter. Peak CL activity was seen 5 min after addition of inducer at 37 degrees C with cells tested within 60 min after collection. Tests to determine precision and reproducibility of the microassay gave a coefficient of variation of 8.5% and 11%, respectively. There was no significant difference between the mean peak CL values for 20 healthy adult donors compared to 14 premature neonates, however, the newborns' CL activity declined more rapidly; CL activity was severely depressed in cells obtained from a patient with chronic granulomatous disease. Results suggest that this microassay provides a simple, rapid, and reliable test of phagocytic function in cases where the amount of blood available for testing is limited.
Authors:
V L Thomas; B A Sanford; M S Driscoll; D T Casto; R S Ramamurthy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  111     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-30     Completed Date:  1988-08-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-32     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
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MeSH Terms
Descriptor/Qualifier:
Humans
Luminescent Measurements
Luminol*
Microchemistry
Neutrophils / physiology*
Phagocytosis*
Pyridazines*
Time Factors
Chemical
Reg. No./Substance:
0/Pyridazines; 521-31-3/Luminol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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