Document Detail


Luminol-dependent chemiluminescence in antibody-sensitized neutrophils stimulated with protein A-bearing staphylococci.
MedLine Citation:
PMID:  3173146     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When mouse polymorphonuclear leukocytes (PMNs) sensitized with rabbit antibody to mouse Ehrlich ascites tumor cells were stimulated by Staphylococcus aureus Cowan I cells, a conspicuous luminol-dependent chemiluminescence was observed in the absence of opsonin. The profile of the chemiluminescence (CL) response evoked by staphylococcal cells from antibody-sensitized PMNs had two peaks. An initial peak, observed within 1 min after stimulation, was sharp and high and a second peak, observed about 5 min after stimulation, was low and extended. The CL response of antibody-sensitized PMNs stimulated by S. aureus Cowan I cells was dose-dependently blocked by preincubation with soluble SpA. Cells of a mutant derived from S. aureus Cowan I strain with trace amounts of cell-bound SpA failed to stimulate the antibody-sensitized PMNs to generate the CL response. The antibody-sensitized PMNs were found to phagocytize SpA-bearing S. aureus cells even in the absence of opsonic serum. These results suggest that the observation presented here might provide a useful tool for the investigation of CL response of PMNs.
Authors:
S Nishihara; K Seki; H Ikigai; S Masuda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  32     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1988  
Date Detail:
Created Date:  1988-10-31     Completed Date:  1988-10-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  535-40     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Jikei University School of Medicine, Tokyo.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Luminescent Measurements
Luminol / pharmacology*
Mice
Mice, Inbred ICR
Microscopy, Electron
Neutrophils / immunology*,  metabolism,  ultrastructure
Phagocytosis
Pyridazines / pharmacology*
Staphylococcal Protein A / immunology*
Staphylococcus aureus / immunology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Pyridazines; 0/Staphylococcal Protein A; 521-31-3/Luminol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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