| Luminol-dependent chemiluminescence in antibody-sensitized neutrophils stimulated with protein A-bearing staphylococci. | |
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MedLine Citation:
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PMID: 3173146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When mouse polymorphonuclear leukocytes (PMNs) sensitized with rabbit antibody to mouse Ehrlich ascites tumor cells were stimulated by Staphylococcus aureus Cowan I cells, a conspicuous luminol-dependent chemiluminescence was observed in the absence of opsonin. The profile of the chemiluminescence (CL) response evoked by staphylococcal cells from antibody-sensitized PMNs had two peaks. An initial peak, observed within 1 min after stimulation, was sharp and high and a second peak, observed about 5 min after stimulation, was low and extended. The CL response of antibody-sensitized PMNs stimulated by S. aureus Cowan I cells was dose-dependently blocked by preincubation with soluble SpA. Cells of a mutant derived from S. aureus Cowan I strain with trace amounts of cell-bound SpA failed to stimulate the antibody-sensitized PMNs to generate the CL response. The antibody-sensitized PMNs were found to phagocytize SpA-bearing S. aureus cells even in the absence of opsonic serum. These results suggest that the observation presented here might provide a useful tool for the investigation of CL response of PMNs. |
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Authors:
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S Nishihara; K Seki; H Ikigai; S Masuda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microbiology and immunology Volume: 32 ISSN: 0385-5600 ISO Abbreviation: Microbiol. Immunol. Publication Date: 1988 |
Date Detail:
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Created Date: 1988-10-31 Completed Date: 1988-10-31 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: JAPAN |
Other Details:
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Languages: eng Pagination: 535-40 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Jikei University School of Medicine, Tokyo. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology Luminescent Measurements Luminol / pharmacology* Mice Mice, Inbred ICR Microscopy, Electron Neutrophils / immunology*, metabolism, ultrastructure Phagocytosis Pyridazines / pharmacology* Staphylococcal Protein A / immunology* Staphylococcus aureus / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Pyridazines; 0/Staphylococcal Protein A; 521-31-3/Luminol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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