Document Detail

Luminol chemiluminescence using xanthine and hypoxanthine as xanthine oxidase substrates.
MedLine Citation:
PMID:  2158934     Owner:  NLM     Status:  MEDLINE    
Luminol chemiluminescence induced by the xanthine or hypoxanthine-O2-xanthine oxidase system is analyzed and compared. Characteristics of the light emission curves were examined considering the conventional reaction scheme for the oxidation of both substrates in the presence of xanthine oxidase. The ratio of the areas of the rate of superoxide production during substrate oxidation to uric acid. The O2-. to uric acid ratio for each substrate can account for differences in xanthine and hypoxanthine-supported light emission, since uric acid is a strong inhibitor of O2-.-dependent luminol chemiluminescence. These results are consistent with a free radical scavenging role for uric acid. A similar but weaker scavenging effect of xanthine may also contribute to the observed differences in chemiluminescent yields between both substrates.
R Radi; H Rubbo; L Thomson; E Prodanov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  8     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  1990  
Date Detail:
Created Date:  1990-06-06     Completed Date:  1990-06-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  121-6     Citation Subset:  IM    
Department of Biochemistry, Faculty of Medicine, University of the Republic, Montevideo, Uruguay.
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MeSH Terms
Dithionite / pharmacology
Hypoxanthines / metabolism*
Luminescent Measurements
Luminol / diagnostic use*
Milk / enzymology
Pyridazines / diagnostic use*
Spectrophotometry, Ultraviolet
Superoxides / metabolism
Uric Acid / metabolism
Xanthine Oxidase / metabolism*
Xanthines / metabolism*
Reg. No./Substance:
0/Hypoxanthines; 0/Pyridazines; 0/Xanthines; 11062-77-4/Superoxides; 14844-07-6/Dithionite; 521-31-3/Luminol; 68-94-0/Hypoxanthine; 69-89-6/Xanthine; 69-93-2/Uric Acid; EC Oxidase

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