| Luminescent probes for ultrasensitive detection of nucleic acids. | |
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MedLine Citation:
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PMID: 20085336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescence-based molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanide-based hybridization probes. These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications. |
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Authors:
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Lev N Krasnoperov; Salvatore A E Marras; Maxim Kozlov; Laura Wirpsza; Arkady Mustaev |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 21 ISSN: 1520-4812 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-05-18 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 319-27 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, 151 Tiernan Hall, University Heights, Newark, New Jersey 07102, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Base Sequence Chelating Agents / chemistry Edetic Acid / analogs & derivatives, chemistry Kinetics Lanthanoid Series Elements / chemistry Luminescent Agents / chemical synthesis, chemistry* Molecular Probes / chemistry, metabolism Nucleic Acids / analysis*, genetics, metabolism Pentetic Acid / chemistry Reproducibility of Results Spectrometry, Fluorescence |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM-30717-21/GM/NIGMS NIH HHS; R01 GM030717-23/GM/NIGMS NIH HHS; R01 MH-079197/MH/NIMH NIH HHS; R01 MH079197-01/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Lanthanoid Series Elements; 0/Luminescent Agents; 0/Molecular Probes; 0/Nucleic Acids; 60-00-4/Edetic Acid; 67-43-6/Pentetic Acid; 869-52-3/triethylenetetraminehexaacetic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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