Document Detail


Luminal hypertonicity and acidity modulate colorectal afferents and induce persistent visceral hypersensitivity.
MedLine Citation:
PMID:  22859365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carbohydrate malabsorption such as in lactose intolerance or enteric infection causes symptoms that include abdominal pain. Because this digestive disorder increases intracolonic osmolarity and acidity by accumulation of undigested carbohydrates and fermented products, we tested whether these two factors (hypertonicity and acidity) would modulate colorectal afferents in association with colorectal nociception and hypersensitivity. In mouse colorectum-pelvic nerve preparations in vitro, afferent activities were monitored after application of acidic hypertonic saline (AHS; pH 6.0, 800 mosM). In other experiments, AHS was instilled intracolonically to mice and behavioral responses to colorectal distension (CRD) measured. Application of AHS in vitro excited 80% of serosal and 42% of mechanically-insensitive colorectal afferents (MIAs), sensitizing a proportion of MIAs to become mechanically sensitive and reversibly inhibiting stretch-sensitive afferents. Acute intracolonic AHS significantly increased expression of the neuronal activation marker pERK in colon sensory neurons and augmented noxious CRD-induced behavioral responses. After three consecutive daily intracolonic AHS treatments, mice were hypersensitive to CRD 4-15 days after the first treatment. In complementary single fiber recordings in vitro, the proportion of serosal class afferents increased at day 4; the proportion of MIAs decreased, and muscular class stretch-sensitive afferents were sensitized at days 11-15 in mice receiving AHS. These results indicate that luminal hypertonicity and acidity, two outcomes of carbohydrate malabsorption, can induce colorectal hypersensitivity to distension by altering the excitability and relative proportions of colorectal afferents, suggesting the potential involvement of these factors in the development of abdominal pain.
Authors:
Jun-Ho La; Bin Feng; Erica S Schwartz; Pablo R Brumovsky; G F Gebhart
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-02
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  303     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-02     Completed Date:  2012-12-18     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G802-9     Citation Subset:  IM    
Affiliation:
Center for Pain Research, Department of Anesthesiology, School of Medicine, University of Pittsburgh, W1402 Biomedical Science Tower, 200 Lothrop St. Pittsburgh, PA 15213, USA. laj@upmc.edu
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MeSH Terms
Descriptor/Qualifier:
Administration, Rectal
Animals
Behavior, Animal / physiology
Colon* / innervation,  physiopathology
Dilatation / psychology
Hypersensitivity* / etiology,  physiopathology
Lactose Intolerance / physiopathology*
Mechanoreceptors / physiology
Mechanotransduction, Cellular / physiology*
Mice
Mice, Inbred C57BL
Physical Stimulation / methods
Rectum* / innervation,  physiopathology
Saline Solution, Hypertonic / administration & dosage
Visceral Afferents / physiology*
Grant Support
ID/Acronym/Agency:
R01 DK093525/DK/NIDDK NIH HHS; R01-DK-093525/DK/NIDDK NIH HHS; R01-NS-035790/NS/NINDS NIH HHS; UL1 RR024153/RR/NCRR NIH HHS; UL1-RR-024153/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Saline Solution, Hypertonic
Comments/Corrections

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