| Luminal chloride modulates rat distal tubule bidirectional bicarbonate flux in vivo. | |
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MedLine Citation:
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PMID: 2347913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of replacing luminal chloride with gluconate on distal tubule bicarbonate transport were studied in vivo in normally fed rats, overnight-fasted rats, and rats made mildly alkalotic by administration of desoxycorticosterone acetate (DOCA). In paired microperfusions of the same tubule with 0 or 55 mM Cl at 25 nl/min, net secretion of bicarbonate by distal tubules of fed rats was inhibited by chloride replacement. Zero chloride perfusion in DOCA rats also resulted in an inhibition of net bicarbonate secretion at 25 nl/min. In contrast, replacement of 45 mM chloride also perfused at 25 nl/min in fasted rats caused an increase in net bicarbonate reabsorption. To further characterize the effects of changes in luminal chloride, experiments were undertaken in fasted rats with 0, 45, and 100 mM chloride-containing solutions perfused at 8 and 25 nl/min. Perfusion with zero Cl resulted in net bicarbonate reabsorption at 8 nl/min that increased markedly with high flow, whereas bicarbonate reabsorption did not change significantly during perfusion at high flow with a 45-mM Cl perfusate. In marked contrast, perfusion with a 100-mM Cl solution resulted in only minimal bicarbonate reabsorption at 8 nl/min with significant secretion observed at high flow. Thus, chloride-free perfusates inhibit bicarbonate secretion and enhance bicarbonate reabsorption, while high chloride perfusates elicit net bicarbonate secretion in usually reabsorbing distal tubules. |
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Authors:
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D Z Levine; D Vandorpe; M Iacovitti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 85 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-07-11 Completed Date: 1990-07-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1793-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, University of Ottawa, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid-Base Equilibrium* Alkalosis / metabolism Animals Bicarbonates / metabolism* Biological Transport Chlorides / metabolism* Desoxycorticosterone / pharmacology Kidney Tubules / metabolism* Kidney Tubules, Distal / metabolism* Male Perfusion Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Bicarbonates; 0/Chlorides; 64-85-7/Desoxycorticosterone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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