| Luminal amino acids acutely decrease intestinal mucosal protein synthesis and protease mRNA in piglets. | |
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MedLine Citation:
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PMID: 10498761 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because parenteral feeding is associated with negative N balance and reduced rates of protein synthesis in intestinal mucosa, we hypothesized that luminal exposure to specific amino acids or energy fuels would stimulate intestinal protein synthesis. We studied the acute effects of luminal nutrients on mucosal protein synthesis in the absence of systemic influences. Multiple jejunal segments constructed in piglets deprived of food overnight (n = 6) were randomly assigned to luminal perfusion with saline, 30 mmol/L amino acid mixture with or without 50 mmol/L glucose, or 30 mmol/L glutamine for 90 min. Protein synthesis was then measured by luminal perfusion with L-[2,6-(3)H]-phenylalanine. Energy substrates (glucose, short-chain fatty acids or beta-hydroxybutyrate) had no effect on mucosal protein synthesis. Relative to saline, a 30 mmol/L amino acid mixture or 30 mmol/L glutamine suppressed mucosal protein synthesis by 20-25% (P < 0.05). On the basis of these surprising results, we speculated that a coordinate reduction of proteolytic processes would be required to maintain positive intestinal N balance. Although intestinal protein catabolism cannot be assessed directly, the 30 mmol/L amino acid mixture acutely suppressed mucosal levels of mRNA encoding ubiquitin, 14-kDa ubiquitin conjugating enzyme and the C9 subunit of the proteasome by 20-30% (P < 0.05), demonstrating the sensitivity of components of the ATP-ubiquitin proteolytic pathway to acute regulation by nutrients. The suppression of protein synthesis by luminal amino acids in the absorptive state might lower intestinal utilization of amino acids to ensure efficient allocation of absorbed nutrients to nonintestinal tissues. |
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Authors:
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O A Adegoke; M I McBurney; S E Samuels; V E Baracos |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of nutrition Volume: 129 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-11-15 Completed Date: 1999-11-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1871-8 Citation Subset: IM |
Affiliation:
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Department of Agricultural, University of Alberta, Edmonton, AB, T6G 2P5 Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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administration & dosage,
pharmacology* Ammonia / pharmacology Analysis of Variance Animals Drug Interactions Endopeptidases / metabolism* Glutamine / pharmacology* Intestinal Mucosa / drug effects*, metabolism Male Protein Biosynthesis* RNA, Messenger / metabolism Swine |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/RNA, Messenger; 56-85-9/Glutamine; 7664-41-7/Ammonia; EC 3.4.-/Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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