Document Detail


LpxI structures reveal how a lipid A precursor is synthesized.
MedLine Citation:
PMID:  23042606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enzymes in lipid metabolism acquire and deliver hydrophobic substrates and products from within lipid bilayers. The structure at 2.55 Å of one isozyme of a constitutive enzyme in lipid A biosynthesis, LpxI from Caulobacter crescentus, has a novel fold. Two domains close around a completely sequestered substrate, UDP-2,3-diacylglucosamine, and open to release products either to the neighboring enzyme in a putative multienzyme complex or to the bilayer. Mutation analysis identifies Asp225 as key to Mg(2+)-catalyzed diphosphate hydrolysis. These structures provide snapshots of the enzymatic synthesis of a critical lipid A precursor.
Authors:
Louis E Metzger; John K Lee; Janet S Finer-Moore; Christian R H Raetz; Robert M Stroud
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-10-07
Journal Detail:
Title:  Nature structural & molecular biology     Volume:  19     ISSN:  1545-9985     ISO Abbreviation:  Nat. Struct. Mol. Biol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-07     Completed Date:  2013-01-16     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101186374     Medline TA:  Nat Struct Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1132-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Biophysics, The University of California San Francisco, San Francisco, California, USA. metzger@msg.ucsf.edu
Data Bank Information
Bank Name/Acc. No.:
PDB/4GGI;  4GGM
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Caulobacter crescentus / enzymology*
Crystallization
DNA Mutational Analysis
Glycolipids / metabolism
Isoenzymes / chemistry
Lipid A / biosynthesis*
Mass Spectrometry
Models, Molecular*
Molecular Sequence Data
Molecular Structure
Protein Conformation*
Protein Folding
Pyrophosphatases / chemistry*,  genetics
Ultracentrifugation
Grant Support
ID/Acronym/Agency:
GM069338/GM/NIGMS NIH HHS; GM24485/GM/NIGMS NIH HHS; GM51310/GM/NIGMS NIH HHS; R01 GM051310/GM/NIGMS NIH HHS; R37 GM024485/GM/NIGMS NIH HHS; U54 GM069338/GM/NIGMS NIH HHS; U54 GM094625/GM/NIGMS NIH HHS; U54GM094625/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Glycolipids; 0/Isoenzymes; 0/Lipid A; 86559-73-1/lipid X; EC 3.6.1.-/Pyrophosphatases; EC 3.6.1.-/UDP-2,3-diacylglucosamine pyrophosphatase
Comments/Corrections

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