Document Detail

Lowering of elevated tissue PCO2 in a hemorrhagic shock rat model after reinfusion of a novel nanobiotechnological polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase that is an oxygen and a carbon dioxide carrier with enhanced antioxidant properties.
MedLine Citation:
PMID:  23347056     Owner:  NLM     Status:  MEDLINE    
Even though erythrocytes transport both oxygen and carbon dioxide, research on blood substitutes has concentrated on the transport of oxygen and its vasoactivity and oxidative effects. Recent study in a hemorrhagic shock animal model shows that the degree of tissue PCO(2) elevation is directly related to mortality rates. We therefore prepared a novel nanobiotechnological carrier for both O(2) and CO(2) with enhanced antioxidant properties. This is based on the use of glutaraldehyde to crosslink stroma free hemoglobin (SFHb), superoxide dismutase (SOD), catalase (CAT) and carbonic anhydrase (CA) to form a soluble PolySFHb-SOD-CAT-CA. It was compared to blood and different resuscitation fluids on the ability to lower elevated tissue PCO(2) in a 2/3 blood volume loss rat hemorrhagic shock model. Sixty minutes of sustained hemorrhagic shock at 30 mm Hg resulted in the increase of tissue PCO(2) to 95 mm ± 3 mmHg from the control level of 55 mm Hg. Reinfusion of whole blood (Hb 15 g/dL with its RBC enzymes) lowered the tissue PCO2 to 72 ± 4.5 mmHg 60 minutes after reinfusion. PolySFHb-SOD-CAT-CA (SFHb 10 g/dL plus additional enzymes) was more effective than whole blood in lowering PCO(2) lowering this to 66.2 ± 3.5 mmHg. Ringer's Lactated solution or polyhemoglobin lowered the elevated PCO2 only slightly to 87 ± 4.5 mmHg and 84.8 ± 1.5 mmHg, respectively. Moreover, ST-elevation for whole blood (Hb 15 g/dL) and PolySFHb-SOD-CAT-CA (Hb 10 g/dL) was respectively 12.8% ± 4% and 13.0% ± 2% of the control 60 minutes after reinfusion. Both are significantly better than those in the Ringer's lactated group and the PolyHb group. In conclusion, this novel approach for blood substitute design has resulted in a novel nanobiotechnological carrier for both O(2) and CO(2) with enhanced antioxidant properties.
Yuzhu Bian; Gao Wei; Thomas M S Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial cells, nanomedicine, and biotechnology     Volume:  41     ISSN:  2169-141X     ISO Abbreviation:  Artif Cells Nanomed Biotechnol     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-25     Completed Date:  2013-07-23     Revised Date:  2014-06-24    
Medline Journal Info:
Nlm Unique ID:  101594777     Medline TA:  Artif Cells Nanomed Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-8     Citation Subset:  IM    
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MeSH Terms
Carbon Dioxide / metabolism
Carbonic Anhydrases / administration & dosage*,  chemical synthesis,  chemistry
Catalase / administration & dosage*,  chemistry
Disease Models, Animal
Hemoglobins / administration & dosage*,  chemistry
Multienzyme Complexes / administration & dosage*,  chemistry
Nanotechnology / methods
Oxygen / metabolism
Rats, Sprague-Dawley
Shock, Hemorrhagic / drug therapy*
Superoxide Dismutase / administration & dosage*,  chemistry
Grant Support
13745//Canadian Institutes of Health Research; MOP13745//Canadian Institutes of Health Research
Reg. No./Substance:
0/Hemoglobins; 0/Multienzyme Complexes; 0/polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase; 142M471B3J/Carbon Dioxide; EC; EC Dismutase; EC Anhydrases; S88TT14065/Oxygen
Erratum In:
Artif Cells Nanomed Biotechnol. 2013 Apr;41(2):144

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