| Low voltage irreversible electroporation induced apoptosis in HeLa cells. | |
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MedLine Citation:
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PMID: 22531519 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: High-voltage electric field pulses can make cell membrane electroporated irreversibly and eliminate malignant cells via necrosis. However, low-voltage is not efficient as that. Aims: This study determined the differential effects of high- and low-voltage electric field pulses on HeLa cells, when the power of low-voltage was enhanced by increasing quantity of pulses. Materials and Methods: Pulses electric fields with permanent frequency (1 Hz) and pulse length (100 μs) were performed on HeLa cells. Voltage and pulse sets (8 pulses/set) were various during treatment. CCK-8 assay was used to detect cell viability. The quantitative determination of apoptosis and necrosis were performed by flow cytometry with Annexin V and PI staining. Transmission electron microscopy was used to observe the ultrastructure of HeLa cells. Caspase-3 and caspase-8, the enzymes in apoptotic pathway, were determined by western blot. Results: The data showed that low-voltage electric field pulses also could make cell irreversible electroporation (IRE) and ablate HeLa cells effectively by induction of apoptosis. The ablating effect due to low-voltage treatments delivered with a greater number of pulses may be as satisfactory as high-voltage, or even preferable because it causes less necrosis and more apoptosis. Conclusions: IRE induced by low voltage with more pulses could ablate HeLa cells effectively as high voltage, and it was preferable that less necrosis and more apoptosis occurred under such condition. |
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Authors:
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Wei Zhou; Zhengai Xiong; Ying Liu; Chenguo Yao; Chengxiang Li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cancer research and therapeutics Volume: 8 ISSN: 1998-4138 ISO Abbreviation: J Cancer Res Ther Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-04-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101249598 Medline TA: J Cancer Res Ther Country: India |
Other Details:
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Languages: eng Pagination: 80-5 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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