Document Detail


Low temperature storage of rhesus monkey spermatozoa and fertility evaluation by intracytoplasmic injection.
MedLine Citation:
PMID:  15910919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective was to develop a sperm freezing procedure suitable for use in the propagation of valuable founder animals by assisted reproductive technologies. Here, we report a comparison of processing methods by measuring the motility of fresh and frozen-thawed rhesus monkey spermatozoa and fertility via intracytoplasmic spermatozoa injection (ICSI) of sibling oocytes. Washed spermatozoa were frozen in straws or in pellets using different cryoprotective media and processed post-thaw with or without a density gradient centrifugation step. Among the four study series, motility post-thaw was improved with density gradient centrifugation (17-24% versus 75%, P<0.01) achieving levels similar to fresh spermatozoa. Spermatozoa injected oocytes (total n=377) were co-cultured on BRL cells and observed for fertilization and development. With spermatozoa frozen in straws in liquid nitrogen vapors, the fertilization rate after ICSI was lower than with fresh spermatozoa (40-44% versus 77-86%, P<0.05), even with the Percoll-enriched fraction that exhibited robust motility. In contrast, somewhat slower freezing of spermatozoa in pellets on dry ice supported fertilization rates (73%) that were similar to the fresh counterpart. Developmental rates of fertilized eggs were similar in all experiments. A total of 106 embryo transfers has resulted in the first primate born after ICSI with F/T ejaculated spermatozoa plus 22 other infants to date. Additionally, a 3-4 h incubation after thawing improved the fertilization rate with spermatozoa from a male with poor post-thaw recovery of sperm motility. In conclusion, an acceptable fertilization rate after ICSI with motile, frozen-thawed primate spermatozoa was observed comparable to that obtained with fresh spermatozoa allowing small quantities of competent spermatozoa to be used with ICSI to facilitate propagation of desirable primate genotypes.
Authors:
Richard R Yeoman; Shoukhrat Mitalipov; Behzad Gerami-Naini; Kevin D Nusser; Don P Wolf
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Theriogenology     Volume:  63     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-24     Completed Date:  2005-08-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2356-71     Citation Subset:  IM    
Affiliation:
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 185th Avenue, Beaverton, Oregon, OR 97006, USA. yeomanr@ohsu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cryopreservation / methods,  veterinary*
Embryo Transfer / veterinary
Female
Fertility*
Hot Temperature
Macaca mulatta*
Male
Pregnancy
Semen Preservation / methods,  veterinary*
Sperm Injections, Intracytoplasmic / veterinary*
Sperm Motility
Grant Support
ID/Acronym/Agency:
A142709//PHS HHS; RR 16030/RR/NCRR NIH HHS; RR12804/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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