Document Detail


Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  22747505     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Unlike other stresses, the physiological significance and molecular mechanisms involved in the yeast cold response are largely unknown. Here, we show that the cell wall integrity (CWI) pathway plays an important role in the growth of S. cerevisiae at low temperatures. Cells lacking the Wsc1p membrane sensor or the MAPKs Bck1p, Mkk1p/Mkk2p or Slt2p exhibited cold sensitivity. However, there was no evidence of either a cold-provoked perturbation of the cell wall or a differential cold expression program mediated by Slt2p. Our results suggest that Slt2p is activated by different inputs in response to nutrient signals and mediates growth control through TORC1-Sch9p and PKA at low temperatures. We found that absence of TOR1 causes cold sensitivity, whereas a ras2Δ mutant shows increased cold growth. Lack of Sch9p alleviates the phenotype of slt2Δ and bck1Δ mutant cells, as well as attenuation of PKA activity by over-expression of BCY1. Interestingly, swi4Δ mutant cells display cold sensitivity, but the phenotype is neither mediated by the Slt2p-regulated induction of Swi4p-responsive promoters nor influenced by osmotic stabilisation. Hence, cold signalling through the CWI pathway has distinct features and might imply still unknown effectors and targets.
Authors:
Isaac Córcoles-Sáez; Lidia Ballester-Tomas; Maria Angeles de la Torre-Ruiz Sr; Jose A Prieto; Francisca Randez-Gil
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-29
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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