Document Detail


Low species diversity and high interindividual variability in faeces of preterm infants as revealed by sequences of 16S rRNA genes and PCR-temporal temperature gradient gel electrophoresis profiles.
MedLine Citation:
PMID:  16819956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Little information regarding the composition of the gut microbiota in preterm infants is available. The purpose of this study was to investigate the bacterial diversity in faeces of preterm infants, using analysis of randomly cloned 16S rRNA genes and PCR-TTGE (temporal temperature gradient gel electrophoresis) profiles, to determine whether noncultivated bacteria represented an important part of the community. The 288 clones obtained from faecal samples of 16 preterm infants were classified into 25 molecular species. All but one molecular species had a cultivated representative in public databases: molecular tools did not reveal any unexplored diversity. The mean number of molecular species per infant was 3.25, ranging from one to eight. There was a high interindividual variability. The main groups encountered were the Enterobacteriaceae family and the genera Enterococcus, Streptococcus and Staphylococcus. Seven preterm infants were colonized by anaerobes and only four by bifidobacteria. TTGE profiles were composed of one to nine bands (mean value: 4.3). Furthermore, 51 of 59 clones (86%) comigrated with a band of the corresponding faecal sample. This study will form a comparative framework for other studies, e.g. on the faecal microbiota of preterm infants with different pathologies or the impact of diet on colonization.
Authors:
Fabien Magne; Michel Abély; Frédérique Boyer; Patrice Morville; Philippe Pochart; Antonia Suau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  57     ISSN:  0168-6496     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-05     Completed Date:  2006-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  128-38     Citation Subset:  IM    
Affiliation:
Laboratoire de biologie, CNAM, Paris, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ083699;  DQ083700;  DQ083701;  DQ083702;  DQ083703;  DQ083704;  DQ083705;  DQ083706;  DQ083707;  DQ083708;  DQ083709;  DQ083710;  DQ083711;  DQ083712;  DQ083713;  DQ083714;  DQ083715;  DQ083716;  DQ083717;  DQ083718;  DQ083719;  DQ083720;  DQ083721;  DQ083722;  DQ083723;  DQ083724;  DQ083725;  DQ083726;  DQ083727;  DQ083728;  DQ083729;  DQ083730;  DQ083731;  DQ083732;  DQ083733;  DQ083734;  DQ083735;  DQ083736;  DQ083737;  DQ083738;  DQ083739;  DQ083740;  DQ083741;  DQ083742;  DQ083743;  DQ083744;  DQ083745;  DQ083746;  DQ083747;  DQ083748;  DQ083749;  DQ083750;  DQ083751;  DQ083752;  DQ083753;  DQ083754;  DQ083755;  DQ083756
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / therapeutic use
Biodiversity
Electrophoresis, Polyacrylamide Gel / methods
Feces / microbiology*
Female
Humans
Infant
Infant, Newborn
Infant, Premature*
Male
Molecular Sequence Data
RNA, Ribosomal, 16S / genetics*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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