| Low-salt diet alters the phospholipid composition of rat colonocytes. | |
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MedLine Citation:
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PMID: 10984084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of low-salt diet on phospholipid composition and remodeling was examined in rat colon which represents a mineralocorticoid target tissue. To elucidate this question, male Wistar rats were fed a low-salt diet and drank distilled water (LS, low-salt group) or saline instead of water (HS, high-salt group) for 12 days before the phospholipid concentration and fatty acid composition of isolated colonocytes were examined. The dietary regimens significantly influenced the plasma concentration of aldosterone which was high in LS group and almost zero in HS group. Plasma concentration of corticosterone was unchanged. When expressed in terms of cellular protein content, a significantly higher concentration of phospholipids was found in LS group, with the exception of sphingomyelin (SM) and phosphatidylserine (PS). Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) accounted for more than 70% of total phospholipids in both groups. A comparison of phospholipid distribution in LS and HS groups demonstrated a higher percentage of PE and a small, but significant, decrease of PC and SM in LS group. The percentage of phosphatidylinositol (PI), PS and cardiolipin (CL) were not affected by mineralocorticoid treatment. With respect to the major phospholipids (PE, PC), a higher level of n-6 polyunsaturated fatty acids (PUFA) and lower levels of monounsaturated fatty acids were detected in PC of LS group. The increase of PUFA predominantly reflected an increase in arachidonic acid by 53%. In comparison to the HS group, oleic acid content was decreased in PC and PE isolated from colonocytes of the LS group. Our data indicate that alterations in phospholipid concentration and metabolism can be detected in rats with secondary hyperaldosteronism. The changes in phospholipid concentration and their fatty acid composition during fully developed effect of low dietary Na+ intake may reflect a physiologically important phenomenon with long-term consequences for membrane structure and function. |
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Authors:
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L Mrnka; O Nováková; F Novák; E Tvrzická; J Pácha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: 49 ISSN: 0862-8408 ISO Abbreviation: Physiol Res Publication Date: 2000 |
Date Detail:
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Created Date: 2000-12-11 Completed Date: 2000-12-22 Revised Date: 2008-04-02 |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: CZECH REPUBLIC |
Other Details:
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Languages: eng Pagination: 197-205 Citation Subset: IM |
Affiliation:
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Institute of Physiology, Czech Academy of Sciences, Prague. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldosterone
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blood Animals Arachidonic Acid / metabolism Colon / cytology, metabolism* Diet, Sodium-Restricted* Fatty Acids / metabolism Male Oleic Acid / metabolism Osmolar Concentration Phosphatidylcholines / metabolism Phosphatidylethanolamines / metabolism Phospholipids / metabolism* Rats Rats, Wistar Sphingomyelins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Sphingomyelins; 112-80-1/Oleic Acid; 506-32-1/Arachidonic Acid; 52-39-1/Aldosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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