Document Detail

Low numbers of interleukin-12-producing cord blood mononuclear cells and immunoglobulin E sensitization in early childhood.
MedLine Citation:
PMID:  15005730     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Successful pregnancies are associated with skewing towards a Th2 cytokine profile. Cytokine responses to allergens can be detected in cord blood mononuclear cells (CBMC), suggesting allergen priming already in utero. OBJECTIVE: To investigate the cytokine profile in CBMC after in vitro stimulation with allergens and to relate the responses to the outcome in terms of allergic disease at 2 years of age. METHODS: CBMC were isolated from 82 children. The responses to ovalbumin (OVA), birch, cat and phytohaemagglutinin (PHA) were investigated by the ELISpot technique. The numbers of IFN-gamma-, IL-4- and IL-12-producing CBMC were counted for each stimulation. The children were followed prospectively; skin prick test (SPT) and RAST towards food and inhalant allergens were assessed at 24 months of age. RESULTS: Sixteen (19.5%) children were classified as IgE sensitized (positive SPT; > or =3 mm and/or RAST; > or =0.35 kUA/L). The numbers of IL-12-producing CBMC after stimulation with birch, OVA and cat were lower among IgE-sensitized children, statistically significant for cat. IFN-gamma-producing cells, did not differ in numbers between the sensitized and non-sensitized children. Children who had atopic eczema/dermatitis syndrome (AEDS) during the observation (n=53) had significantly lower numbers of IFN-gamma-producing CBMC after stimulation with OVA and cat than their non-AEDS counterparts. CONCLUSIONS: Although the numbers of infants in our study are limited our data suggest that a low number of IL-12-producing CBMC is associated with IgE sensitization during early childhood and that a reduced number of IFN-gamma-producing CBMC promotes the development of AEDS during the first 2 years of life.
C Nilsson; A-K Larsson; A Höglind; S Gabrielsson; M Troye Blomberg; G Lilja
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  34     ISSN:  0954-7894     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-09     Completed Date:  2004-05-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  373-80     Citation Subset:  IM    
Department of Pediatrics, Sachs' Children's Hospital, Karolinska Institutet, Stockholm, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Allergens / administration & dosage
Asthma / immunology
Child, Preschool
Dermatitis, Atopic / immunology
Fetal Blood / immunology*
Follow-Up Studies
Food Hypersensitivity / immunology
Hypersensitivity / diagnosis,  immunology*
Immunoglobulin E / immunology*
Interferon-gamma / immunology
Interleukin-12 / immunology*
Interleukin-4 / immunology
Leukocyte Count
Leukocytes, Mononuclear / immunology*
Prospective Studies
Skin Tests
Reg. No./Substance:
0/Allergens; 187348-17-0/Interleukin-12; 207137-56-2/Interleukin-4; 37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  New pregnancies and loss of allergy.
Next Document:  The relation of markers of fetal growth with asthma, allergies and serum immunoglobulin E levels in ...