| Low number of memory B cells in the salivary glands of patients with primary Sjögren's syndrome. | |
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MedLine Citation:
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PMID: 22849322 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have previously shown that patients with primary Sjögren's Syndrome (pSS) show a significant reduction of autoantigen specific CD27(+) memory B cells and an abnormally elevated level of autoantibody producing plasma cells in peripheral blood (PB) compared to controls. Because both memory B cells and plasma cells have been detected in salivary glands (SG) of pSS patients, we aimed to study the B cell pattern in SG biopsies. Double immunohistochemical staining of CD20 and CD27 was carried out on paraffin-embedded SG tissue from 10 pSS patients to distinguish CD20(+)/CD27(+) memory B cells, and identify the CD20(+) glandular B cell zones (BCZ). Given that plasma blasts and plasma cells are CD27(++) and CD20(-), additional CD138 single staining of serial sections allowed the distinction of CD27(++)/CD138(-) plasma blasts located within the BCZ from CD27(++)/CD138(+) plasma cells that were found mostly on the periphery of the BCZ and also observed interstitially. Both BCZ and the memory B cell populations were then quantified. Contrary to what has been reported earlier through immunoflourescent staining of memory B cells in SG tissue, we have shown that there is a low number of memory B cells located within the glandular BCZ. Plasma blasts and plasma cells, however, were more abundant in the SG. Together our findings suggest that these low numbers of memory B cells in both PB and SG of pSS patients may be the result of activation of these cells into plasma cells at the site of inflammation. |
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Authors:
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Lara A Aqrawi; Karl Albert Brokstad; Kjerstin Jakobsen; Roland Jonsson; Kathrine Skarstein |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-1 |
Journal Detail:
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Title: Autoimmunity Volume: - ISSN: 1607-842X ISO Abbreviation: Autoimmunity Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8900070 Medline TA: Autoimmunity Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Broegelmann Research Laboratory, The Gade Institute , Bergen , Norway. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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