Document Detail


Low molecular weight polyethylenimine for efficient transfection of human hematopoietic and umbilical cord blood-derived CD34+ cells.
MedLine Citation:
PMID:  15953679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With the emerging role of hematopoietic stem cells as potential gene and cell therapy vehicles, there is an increasing need for safe and effective nonviral gene delivery systems. Here, we report that gene transfer and transfection efficiency in human hematopoietic and cord blood CD34+ cells can be enhanced by the use of low molecular weight polyethylenimine (PEI). PEIs of various molecular weights (800-750,000) were tested, and our results showed that the uptake of plasmid DNA by hematopoietic TF-1 cells depended on the molecular weights and the N/P ratios. Treatment with PEI 2K (m.w. 2000) at an N/P ratio of 80/1 was most effective, increasing the uptake of plasmid DNA in TF-1 cells by 23-fold relative to Lipofectamine 2000. PEI 2K-enhanced transfection was similarly observed in hematopoietic K562, murine Sca-1+, and human cord blood CD34+ cells. Notably, in human CD34+ cells, a model gene transferred with PEI 2K showed 21,043- and 513-fold higher mRNA expression levels relative to the same construct transfected without PEI or with PEI 25 K, respectively. Moreover, PEI 2K-treated TF-1 and human CD34+ cells retained good viability. Collectively, these results indicate that PEI 2K at the optimal N/P ratio might be used to safely enhance gene delivery and transfection of hematopoietic and human CD34+ stem cells.
Authors:
Jee-Young Shin; Dongchul Suh; Jung Mogg Kim; Han-Gon Choi; Jung Ae Kim; Jung Jae Ko; Yong Bok Lee; Jin-Seok Kim; Yu-Kyoung Oh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-05-31
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1725     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-03     Completed Date:  2005-11-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  377-84     Citation Subset:  IM    
Affiliation:
CHA Research Institute, College of Medicine, Pochon CHA University, Pochon, Kyonggi-do 487-800, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD34 / blood*
Cell Survival
DNA / administration & dosage
Fetal Blood / immunology
Hematopoietic Stem Cells / physiology*
Humans
Interleukin-2 / biosynthesis,  genetics
K562 Cells
Molecular Weight
Plasmids / genetics
Polyethyleneimine / administration & dosage,  chemistry*,  toxicity
Transfection / methods*
Chemical
Reg. No./Substance:
0/Antigens, CD34; 0/Interleukin-2; 9002-98-6/Polyethyleneimine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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