Document Detail


Low molecular weight phosphotyrosine protein phosphatase from PC12 cells. Purification, some properties and expression during neurogenesis in vitro and in vivo.
MedLine Citation:
PMID:  12798350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purification and partial characterization of low molecular weight phosphotyrosine phosphatase (LMW-PTP) was reported for the first time in PC12 cells. In addition, the expression levels during neuronal phenotype induction by nerve growth factor (NGF) and during neurogenesis in chick embryos were investigated. LMW-PTP was purified to homogeneity and showed a single band of about 18 kDa with sodium dodecyl sulfate polyacrylamide gel electrophoresis. A native molecular mass of 20.1 kDa was determined by gel filtration on Sephadex G-75 column. The LMW-PTP from PC12 cells displays structural and biochemical characteristics similar to the enzyme isolated for normal tissues. It was specifically immunoprecipitated by an affinity purified antibody directed against the bovine liver enzyme. The enzyme is present in the cytosolic and cytoskeletal cell compartment where is tyrosine phosphorylated. Time course expression of LMW-PTP in PC12 cells was investigated after NGF treatment and showed an increase of about 30% in the basal level of LMW-PTP from 0 to 72 h. These changes were related to the appearance in PC12 cells of neuronal processes and to a decrease in cell proliferation. An increase of the LMW-PTP expression was also observed in vivo during chick embryo neurogenesis from 8-day-old embryos to adult chicks. The protein level, assayed by immunoblotting, increases from 14-day-old embryos to the hatched chicks reaching the adult levels within the first week after birth. These data indicate that the neurogenesis process is accompanied by a physiological increment of LMW-PTP expression in vitro and in vivo.
Authors:
Livia Lucentini; Stefania Fulle; Claudia Ricciolini; Hovirag Lancioni; Fausto Panara
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The international journal of biochemistry & cell biology     Volume:  35     ISSN:  1357-2725     ISO Abbreviation:  Int. J. Biochem. Cell Biol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-06-11     Completed Date:  2003-09-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9508482     Medline TA:  Int J Biochem Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1378-87     Citation Subset:  IM    
Affiliation:
Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Via Pascoli, I-06123 Perugia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / embryology,  enzymology*
Cell Differentiation
Chick Embryo
Cytoskeleton / enzymology
Cytosol / enzymology
Isoenzymes / chemistry,  isolation & purification*
PC12 Cells
Protein Tyrosine Phosphatases / chemistry,  isolation & purification*
Proto-Oncogene Proteins*
Rats
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Proto-Oncogene Proteins; EC 3.1.3.48/Acp1 protein, rat; EC 3.1.3.48/Protein Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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