Document Detail


Low molecular weight heparins: structural differentiation by bidimensional nuclear magnetic resonance spectroscopy.
MedLine Citation:
PMID:  17629844     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Individual low molecular weight heparins (LMWHs) exhibit distinct pharmacological and biochemical profiles because of manufacturing differences. Correlation of biological properties with particular structural motifs is a major challenge in the design of new LMWHs as well as in the development of generic versions of proprietary LMWHs. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy permits identification and quantification of structural peculiarities of LMWH preparations. In this article, heteronuclear single quantum coherence spectroscopy, previously used to determine variously substituted monosaccharide components of heparan sulfate (HS) and HS-like glycosaminoglycan mimics, has been applied to the structural characterization of three commercially available LMWHs (enoxaparin, dalteparin, and tinzaparin). Relevant residues belonging to the parent heparin, as well as minor residues generated by each depolymerization procedure, have been characterized and quantified. The use of a high-sensitivity NMR spectrometer (600 MHz equipped with cryoprobe) allowed the accurate quantification of residues with sensitivity better than 1 to 2%.
Authors:
Marco Guerrini; Sara Guglieri; Annamaria Naggi; Ram Sasisekharan; Giangiacomo Torri
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Seminars in thrombosis and hemostasis     Volume:  33     ISSN:  0094-6176     ISO Abbreviation:  Semin. Thromb. Hemost.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-16     Completed Date:  2007-09-27     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0431155     Medline TA:  Semin Thromb Hemost     Country:  United States    
Other Details:
Languages:  eng     Pagination:  478-87     Citation Subset:  IM    
Affiliation:
Institute for Chemical and Biochemical Research G. Ronzoni, Via G. Colombo 81, 20133 Milan, Italy. guerrini@ronzoni.it
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MeSH Terms
Descriptor/Qualifier:
Heparin, Low-Molecular-Weight / chemistry*
Magnetic Resonance Spectroscopy*
Molecular Structure
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
1R01 HL 080278-01/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Heparin, Low-Molecular-Weight

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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