Document Detail


Low molecular weight heparin enhances prostacyclin production by cultured human endothelial cells.
MedLine Citation:
PMID:  1667624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Confluent cultures of vascular endothelial cells derived from the human umbilical vein were incubated in a serum-free medium in the presence of low molecular weight heparin (LMWH) with molecular weights of 4000-6000 dalton (Da), or of unfractionated heparin (UFH) with average molecular weight 12,000 Da, and prostacyclin production was determined by radioimmunoassay for 6-keto-prostaglandin F1 alpha, the stable metabolite of prostacyclin. LMWH at 1 U/ml as anti-factor Xa activity significantly increased prostacyclin production after 6h or longer; however, UFH at 1 USP U/ml did not induce such a significant change. The LMWH-induced increase in prostacyclin production occurred at 0.1 U/ml and above after 6 h of treatment. Since prostacyclin is both a potent inhibitor of platelet aggregation and a vasodilator, it was suggested that the increased endothelial cell prostacyclin production induced by LMWH may be a component of the anticoagulant activity of the drug.
Authors:
T Kaji; C Yamamoto; M Sakamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  39     ISSN:  0009-2363     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-06-19     Completed Date:  1992-06-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  3368-9     Citation Subset:  IM    
Affiliation:
Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Endothelium, Vascular / drug effects,  metabolism*
Epoprostenol / biosynthesis*
Heparin, Low-Molecular-Weight / pharmacology*
Humans
Chemical
Reg. No./Substance:
0/Heparin, Low-Molecular-Weight; 35121-78-9/Epoprostenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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