Document Detail


Low-molecular-mass thiol compounds from a free-living highly pathogenic amoeba, Naegleria fowleri.
MedLine Citation:
PMID:  12630909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acid extracts labelled with the fluorescent reagent monobromobimane and separated by HPLC have enabled the detection of low-molecular-mass thiol compounds in Naegleria fowleri for the first time. The amounts detected are expressed in nmol/1 x 10(6) trophozoites cultivated at various stages of growth in the appropriate culture medium. N. fowleri is a highly pathogenic free-living amoeba, in which we found important thiol compounds, some of them in their reduced and oxidized forms. Unlike cysteine and glutathione, a number of these are not represented in normal human lymphocytes. Some of these thiol compounds from Naegleria must have their respective disulphide reductases, although the presence of thiol-disulphide exchange reactions must be considered. Ovothiol A, with antioxidant properties, is an example of a compound that is kept reduced by trypanothione in trypanosomatids, although no disulphide reductase for ovothiol A has yet been discovered. In our case we were unable to detect this biothiol in Naegleria. The presence of thiol compounds that seem to be particular to this pathogen and which are not present in human lymphocytes opens the possibility of searching for disulphide-reducing enzymes that can serve as drug targets.
Authors:
Raúl N Ondarza; Angélica Iturbe; Eva Hernández; Gerardo Hurtado
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  37     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-12     Completed Date:  2003-11-06     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  195-204     Citation Subset:  IM    
Affiliation:
Center of Research on Infectious Diseases, National Institute of Public Health, Cuernavaca, Morelos, Mexico 62508. ondarza@servidor.unam.mx
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MeSH Terms
Descriptor/Qualifier:
Animals
Bicyclo Compounds
Cells, Cultured
Chromatography, High Pressure Liquid / methods*
Microchemistry / methods*
Molecular Weight
Naegleria fowleri / chemistry,  growth & development*,  metabolism*
Spectrometry, Fluorescence / methods
Sulfhydryl Compounds / analysis*,  metabolism*
Chemical
Reg. No./Substance:
0/Bicyclo Compounds; 0/Sulfhydryl Compounds; 71418-44-5/monobromobimane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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