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Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells.
MedLine Citation:
PMID:  22865122     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the cells. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Morphological appearances of the cells were observed using scanning electron microcopy after 24 and 96 h of incubation. At 24 and 96 h, the control group had a higher cell proliferation than 0.06 and 0.2 mT PEMF groups (p = 0.001). At 96 h, 0.2 mT PEMF group had higher cell proliferation rate than 0.06 mT PEMF and LLLT groups (p = 0.001). The cell count and cell viability in 0.2 mT PEMF group were higher than the 0.06-mT PEMF and LLLT groups, although these differences were not statistically significant at 96 h (p > 0.05). At 24 and 96 h, cell viability in the control group was higher than the test groups. Alkaline phosphatase levels of the groups were comparable in both time intervals (p > 0.05). 0.2 mT PEMF application on osteoblast-like cells led to cell proliferation and differentiation better than 0.06 mT PEMF and LLLT at 808 nm, although a remarkable effect of both PEMF and LLLT could not be detected. The ALP activity of 0.2 and 0.06 mT PEMF and LLLT were comparable.
Authors:
Yusuf Emes; Kivanç Akça; Buket Aybar; Serhat Yalçın; Yeliz Cavuşoğlu; Uğur Baysal; Halim Işsever; Belir Atalay; Pervin Vural; Mine Ergüven; Murat Cavit Cehreli; Ayhan Bilir
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-5
Journal Detail:
Title:  Lasers in medical science     Volume:  -     ISSN:  1435-604X     ISO Abbreviation:  Lasers Med Sci     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8611515     Medline TA:  Lasers Med Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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