Document Detail


Low-flow ischemia leads to translocation of canine heart GLUT-4 and GLUT-1 glucose transporters to the sarcolemma in vivo.
MedLine Citation:
PMID:  9008459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Myocardial ischemia increases heart glucose utilization in vivo. However, whether low-flow ischemia leads to the translocation of glucose transporter (GLUT)-4 and/or GLUT-1 to the sarcolemma in vivo is unknown.
METHODS AND RESULTS: In a canine model, we evaluated myocardial glucose metabolism in vivo and the distribution of GLUT-4 and GLUT-1 by use of immunoblotting of sarcolemma and intracellular membranes and immunofluorescence localization with confocal microscopy. In vivo glucose extraction increased fivefold (P < .001) and was associated with net lactate release in the ischemic region. Ischemia led to an increase in the sarcolemma content of both GLUT-4 (15 +/- 2% to 30 +/- 3%, P < .02) and GLUT-1 (41 +/- 4% to 58 +/- 3%, P < .03) compared with the nonischemic region and to a parallel decrease in their intracellular contents. Immunofluorescence demonstrated the presence of both GLUT-4 and GLUT-1 on cardiac myocytes. GLUT-1 had a more prominent cell surface pattern than GLUT-4, which was primarily intracellular in the nonischemic region. However, significant GLUT-4 surface labeling was found in the ischemic region.
CONCLUSIONS: Translocation of the insulin-responsive GLUT-4 transporter from an intracellular storage pool to the sarcolemma occurs in vivo during acute low-flow ischemia. GLUT-1 is also present in an intracellular storage pool from which it undergoes translocation to the sarcolemma in response to ischemia. These results indicate that both GLUT-1 and GLUT-4 are important in ischemia-mediated myocardial glucose uptake in vivo.
Authors:
L H Young; Y Renfu; R Russell; X Hu; M Caplan; J Ren; G I Shulman; A J Sinusas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation     Volume:  95     ISSN:  0009-7322     ISO Abbreviation:  Circulation     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-27     Completed Date:  1997-02-27     Revised Date:  2013-11-21    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  415-22     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Dogs
Fluorescent Antibody Technique
Glucose Transporter Type 1
Glucose Transporter Type 4
Heart / physiopathology
Intracellular Membranes / metabolism
Monosaccharide Transport Proteins / metabolism*
Muscle Proteins*
Myocardial Ischemia / metabolism*,  pathology,  physiopathology*
Myocardium / metabolism*,  pathology
Regional Blood Flow
Sarcolemma / metabolism*
Subcellular Fractions / metabolism
Tissue Distribution
Grant Support
ID/Acronym/Agency:
5T32-DK-07058-20/DK/NIDDK NIH HHS; P30-DK-45735/DK/NIDDK NIH HHS; R01 DK040936/DK/NIDDK NIH HHS; R01-DK-40936/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Glucose Transporter Type 1; 0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins
Comments/Corrections
Comment In:
Circulation. 1997 Jan 21;95(2):313-5   [PMID:  9008441 ]
Circulation. 1997 Nov 18;96(10):3810-1   [PMID:  9396512 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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