Document Detail


Low flow enhances platelet activation after acute experimental arterial injury.
MedLine Citation:
PMID:  9620144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Vascular smooth muscle cell (VSMC) proliferation and migration to the subintima or intimal hyperplasia (IH) occur after arterial injury and are thought to be induced by mitogenic factors released from activated platelets. Because low flow (LF) and shear have been attributed to the localization and progression of IH, we postulated that hemodynamic factors may regulate the degree of platelet activation, as measured by plasma thromboxane B2 (TXB2) and platelet-derived growth factor-AB (PDGF-AB) release at regions of experimental arterial injury.
METHODS: The right common carotid artery (CCA) was subjected to balloon injury in 18 New Zealand White male rabbits. Flow in the injured CCA was reduced by out-flow ligation (LF group, n = 6) or increased by ligation of the left CCA (high flow [HF] group, n = 6). In six other animals, flow was preserved (normal flow [NF] group). Mean blood flow and pressure in the right CCA were measured thereafter at 10 and 30 minutes. Plasma TXB2 and PDGF-AB levels were determined with the enzyme-linked immunosorbent assay method in each animal with blood samples taken systematically before injury (baseline) and in the distal CCA at similar time points.
RESULTS: At 10 minutes, mean blood flow was reduced from 20 +/- 2 ml/min in the NF group to 7 +/- 1 ml/min in the LF group animals (p < 0.01) and increased to 32 +/- 2 ml/min in the HF group animals (p < 0.05). Mean arterial blood pressure did not differ among the groups. Hemodynamic parameters were similar at 10 and 30 minutes. TXB2 levels were more than fourfold greater in the LF group than in the HF and NF groups at both time points (p < 0.05). In addition, there was a twofold increase in plasma PDGF-AB level at 10 minutes in the LF group compared with baseline levels (p < 0.05).
CONCLUSION: Platelet activation at regions of acute vascular injury was determined to be flow dependent. Upregulated platelet activity in low flow conditions may be due to increased platelet exposure time to subendothelial collagen and is greatly attenuated if normal or increased flow is present.
Authors:
H S Bassiouny; R H Song; H Kocharyan; E Kins; S Glagov
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of vascular surgery     Volume:  27     ISSN:  0741-5214     ISO Abbreviation:  J. Vasc. Surg.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-29     Completed Date:  1998-06-29     Revised Date:  2012-10-03    
Medline Journal Info:
Nlm Unique ID:  8407742     Medline TA:  J Vasc Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  910-8     Citation Subset:  IM    
Affiliation:
Department of Surgery, The University of Chicago, Ill 60637, USA.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Balloon Dilation / adverse effects
Blood Flow Velocity / physiology
Blood Platelets / secretion
Blood Pressure / physiology
Carotid Artery Injuries*
Carotid Artery, Common / pathology
Cell Division
Cell Movement
Collagen
Disease Progression
Enzyme-Linked Immunosorbent Assay
Hemorheology
Hyperplasia
Male
Mitogens / secretion
Muscle, Smooth, Vascular / pathology
Platelet Activation / physiology*
Platelet-Derived Growth Factor / analysis,  secretion
Rabbits
Recombinant Proteins
Regional Blood Flow / physiology
Thromboxane B2 / blood
Time Factors
Tunica Intima / injuries,  pathology
Up-Regulation
Grant Support
ID/Acronym/Agency:
R01-HL55296-01/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Mitogens; 0/Platelet-Derived Growth Factor; 0/Recombinant Proteins; 0/platelet-derived growth factor AB; 54397-85-2/Thromboxane B2; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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