| Low expression of human beta-defensin 1 in duodenum of celiac patients is partially restored by a gluten-free diet. | |
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MedLine Citation:
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PMID: 20128731 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Human beta-defensins (hBDs) are small cationic, widely expressed proteins involved in innate immunity that exert strong bactericidal activity toward various pathogens. However, the role of hBDs in various diseases to which bacterial infection add severity, as it is in celiac disease (CD), is not yet clear. We analyzed the expression of the hBD1, hBD2, hBD3 and hBD4 genes in patients with CD during the active phase and after remission following a gluten-free diet to determine their role in development and relapse of CD. METHODS: We studied 21 unrelated adults with CD (confirmed by anti-thyroglobulin antibodies and intestinal biopsy); 14 were evaluated at diagnosis, before diet modification, and seven after 2 years of a gluten-free diet. Thirty-six unrelated adults served as controls. We analyzed the mRNA expression of hBD1, 2, 3 and 4 on biopsy samples of duodenum obtained from all patients during endoscopy for diagnostic purposes. We used real-time polymerase chain reaction with TaqMan probes and obtained gene expression data using the delta-Ct method. RESULTS: hBD1 mRNA was significantly lower in patients with active CD compared with patients on diet modification, whereas the mRNA levels of the other three defensins did not differ significantly between the two subgroups. Interestingly, the gluten-free diet restored only partially hBD1 expression as compared to a normal group of celiac-free subjects. CONCLUSIONS: Our data reinforce the evidence that hBD1 expression is greatly reduced in the duodenum of patients with active CD. It also strengthens the concept that reduced activity of immune peptides may predispose individuals to bacterial proliferation that contributes to the pathogenesis of CD. |
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Authors:
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Mariano Intrieri; Assunta Rinaldi; Olga Scudiero; Giovanni Autiero; Giuseppe Castaldo; Gerardo Nardone |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-19 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 489-92 Citation Subset: IM |
Affiliation:
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Dipartimento di Biochimica e Biotecnologie Mediche, Universit? degli Studi di Napoli Federico II, Naples, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Autoantibodies / analysis, immunology Biopsy Celiac Disease / diagnosis, genetics, metabolism* Diet, Gluten-Free* Duodenum / metabolism* Humans RNA, Messenger / metabolism Recurrence beta-Defensins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/DEFB1 protein, human; 0/RNA, Messenger; 0/anti-thyroglobulin; 0/beta-Defensins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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