Document Detail

Low electroosmotic flow measurement by tilting microchip.
MedLine Citation:
PMID:  18499115     Owner:  NLM     Status:  MEDLINE    
A novel method for low electroosmotic flow (EOF) rates measurement by tilting microchip which based upon the hydrostatic pressure conception and sampling zone method is described. Sampling zone could be detected in the tilting microchip but not in non-tilting one due to the hydrostatic pressure driven. The method is fulfilled to calculate low EOF rates by detecting the liquid flow velocity driven by hydrostatic pressure, and difference between the apparent mobility of the migrating analyte in two modes is caused by the effect of hydrostatic pressure. And then the EOF rates in unknown low EOF microchip can be calculated. Different microchannels modified with bovine serum albumin (BSA), myoglobin (MB) and polyvinyl alcohol (PVA) were used to verify the method, the EOF rate value was 1.73+/-0.03, 1.21+/-0.05, 0.34+/-0.04 x 10(-4)cm(2) V(-1)s(-1), respectively. The results obtained by the proposed method were agreed well with conventional methods.
Fang Zhou; Wei Wang; Wen-Ya Wu; Jian-Rong Zhang; Jun-Jie Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-08
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1194     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-02     Completed Date:  2008-09-02     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  221-4     Citation Subset:  IM    
Key Laboratory of Analytical Chemistry for Life Sciences (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
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MeSH Terms
Electroosmosis / methods*
Electrophoresis, Microchip / methods*
Myoglobin / chemistry
Polyvinyl Alcohol / chemistry
Reproducibility of Results
Serum Albumin, Bovine / chemistry
Reg. No./Substance:
0/Myoglobin; 0/Serum Albumin, Bovine; 9002-89-5/Polyvinyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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