| Low doses of hydrogen peroxide impair glucose-stimulated insulin secretion via inhibition of glucose metabolism and intracellular calcium oscillations. | |
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MedLine Citation:
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PMID: 19800636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The inhibitory effect of hydrogen peroxide (H(2)O(2)) on glucose-stimulated insulin secretion was previously reported. However, the precise mechanism involved was not systematically investigated. In this study, the effects of low concentrations of H(2)O(2) (5-10 micromol/L) on glucose metabolism, intracellular calcium ([Ca(2+)](i)) oscillations, and dynamic insulin secretion in rat pancreatic islets were investigated. Low concentrations of H(2)O(2) impaired insulin secretion in the presence of high glucose levels (16.7 mmol/L). This phenomenon was observed already after 2 minutes of exposure to H(2)O(2). Glucose oxidation and the amplitude of [Ca(2+)](i) oscillations were dose-dependently suppressed by H(2)O(2). These findings indicate that low concentrations of H(2)O(2) reduce insulin secretion in the presence of high glucose levels via inhibition of glucose metabolism and consequent impairment in [Ca(2+)](i) handling. |
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Authors:
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Eduardo Rebelato; Fernando Abdulkader; Rui Curi; Angelo R Carpinelli |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-02 |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 59 ISSN: 1532-8600 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: United States |
Other Details:
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Languages: eng Pagination: 409-13 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP 05508-000, Brazil. rebelato@icb.usp.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism* Calcium Signaling / drug effects Dose-Response Relationship, Drug Female Glucose / metabolism*, pharmacology* Hydrogen Peroxide / pharmacology* Indicators and Reagents Insulin / metabolism* Islets of Langerhans / drug effects, metabolism Oxidants / pharmacology* Rats |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Oxidants; 11061-68-0/Insulin; 50-99-7/Glucose; 7440-70-2/Calcium; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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