Document Detail

Low-dose radiation response of primary keratinocytes and fibroblasts from patients with cervix cancer.
MedLine Citation:
PMID:  17316072     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to examine, using the micronucleus (MN) assay, the low-dose radiation response of normal skin cells from cancer patients and to determine whether the hyper-radiosensitivity (HRS)-like phenomenon occurs in cells of these patients. Primary skin fibroblasts and keratinocytes derived from 40 patients with cervix cancer were studied. After in vitro gamma irradiation with single doses ranging from 0.05 to 4 Gy, MN induction was assessed. For each patient, the linear-quadratic (LQ) model and the induced repair (IR) model were fitted over the whole data set. In fits of the IR model, an HRS-like response after low doses (seen as the deviation over the LQ curve) was demonstrated for the fibroblasts of two patients and for the keratinocytes of four other patients. The alpha(s)/alpha(r) ratio for the six patients ranged from 2.7 to 15.4, whereas the values of the parameter d(c) ranged from 0.13 to 0.36 Gy. No relationship was observed between chromosomal radiosensitivity of fibroblasts and keratinocytes derived from the same donor in the low-dose (0.1-0.25 Gy) region. In conclusion, the fact that low-dose chromosomal hypersensitivity was observed for cells of only six of the patients studied suggests that it is not a common finding in human normal cells and can represent an individual characteristic.
Dorota Słonina; Beata Biesaga; Krzysztof Urbański; Zbigniew Kojs
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  167     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-23     Completed Date:  2007-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  251-9     Citation Subset:  IM; S    
Department of Applied Radiobiology, Centre of Oncology, Garncarska 11, 31-115 Kraków, Poland.
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MeSH Terms
Cell Proliferation / radiation effects
Chromosomes, Human / genetics
Fibroblasts / metabolism,  pathology*,  radiation effects*
Keratinocytes / metabolism,  pathology*,  radiation effects*
Middle Aged
Tumor Cells, Cultured
Uterine Cervical Neoplasms / genetics,  pathology*

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