Document Detail


Low-dose radiation employed in diagnostic imaging causes genetic effects in cultured cells.
MedLine Citation:
PMID:  20860496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Exposure to environmental, diagnostic, and occupational sources of radiation frequently involves low doses. Although these doses have no immediately noticeable impact on human health there is great interest in their long-term biological effects.
PURPOSE: To assess immediate and time-delayed DNA damage in two cell lines exposed to low doses of ionizing radiation by using the comet assay and micronucleus test, and to compare these two techniques in the analysis of low-dose induced genotoxicity.
MATERIAL AND METHODS: CHO and MRC-5 cells were exposed to 50 milliSievert (mSv) of ionizing radiation and assayed immediately after irradiation and at 16 or 12 passages post-irradiation, respectively. Comet assay and micronucleus test were employed.
RESULTS: The comet assay values observed in 50 mSv-treated cells were significantly higher than in the control group for both sample times and cell lines (P < 0.001). Micronuclei frequencies were higher in treated cells than in the control group (P < 0.01, CHO cells passage 16; P < 0.05, MRC-5 cells immediately after exposure; P < 0.01 MRC-5 cells passage 12). Correlation analysis between the two techniques was statistically significant (correlation coefficient 0.82, P < 0.05 and correlation coefficient 0.86, P < 0.05 for CHO and MRC-5 cells, respectively). Cells scored at passages 12 or 16 showed more damage than those scored immediately after exposure in both cell lines (no statistically significant differences).
CONCLUSION: Cytomolecular and cytogenetic damage was observed in cells exposed to very low doses of X-rays and their progeny. A single low dose of ionizing radiation was sufficient to induce such response, indicating that mammalian cells are exquisitely sensitive to it. Comet and micronucleus assays are sensitive enough to assess this damage, although the former seems to be more efficient.
Authors:
María V Ponzinibbio; Cintia Crudeli; Pilar Peral-García; Analía Seoane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta radiologica (Stockholm, Sweden : 1987)     Volume:  51     ISSN:  1600-0455     ISO Abbreviation:  Acta Radiol     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8706123     Medline TA:  Acta Radiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1028-33     Citation Subset:  IM    
Affiliation:
IGEVET (Instituto de Genética Veterinaria), UNLP-CONICET, La Plata, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells / radiation effects*
Cell Survival / radiation effects
Cells, Cultured / radiation effects*
Comet Assay
Cricetinae
Cricetulus
Cytogenetics
DNA Damage*
Diagnostic Imaging / adverse effects
Dose-Response Relationship, Radiation
Fibroblasts / radiation effects*
Micronucleus Tests
Microscopy, Fluorescence
Radiation Dosage*
Radiation Tolerance
Radiation, Ionizing*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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