Document Detail

Low-dose oral methotrexate induces apoptosis of tissue eosinophils in bullous pemphigoid.
MedLine Citation:
PMID:  18480918     Owner:  NLM     Status:  MEDLINE    
We have shown previously that eosinophils are abundant in blood and tissue in bullous pemphigoid and that they rapidly disappear, without sign of necrosis, during methotrexate therapy. The aim of the present study was to investigate the role of apoptosis in this phenomenon. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling method for detecting apoptosis was used, combined with immunohistochemical detection of eosinophils before and during oral methotrexate. The numbers of apoptotic eosinophils were compared using light microscopy. Eosinophil apoptosis was significantly higher in skin after one week of methotrexate therapy and the number of eosinophils was reduced. The results indicate that methotrexate therapy induces apoptosis of the tissue eosinophils in bullous pemphigoid.
Kristina Dahlman-Ghozlan; Jean-Paul Ortonne; Philippe Bahadoran; Anne Spadafora; Eija Stephansson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta dermato-venereologica     Volume:  88     ISSN:  0001-5555     ISO Abbreviation:  Acta Derm. Venereol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-15     Completed Date:  2008-09-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370310     Medline TA:  Acta Derm Venereol     Country:  Sweden    
Other Details:
Languages:  eng     Pagination:  219-22     Citation Subset:  IM    
Department of Dermatology, Karolinska University Hospital, Solna, Stockholm, Sweden and 2Hospital Archet II, INSERM U385, Nice, France.
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MeSH Terms
Administration, Oral
Apoptosis / drug effects*
Eosinophils / drug effects,  pathology*
Immunosuppressive Agents / administration & dosage*
In Situ Nick-End Labeling
Methotrexate / administration & dosage*
Pemphigoid, Bullous / drug therapy,  pathology*
Skin / pathology
Reg. No./Substance:
0/Immunosuppressive Agents; 59-05-2/Methotrexate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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