Document Detail


Low dose hyper-radiosensitivity is eliminated during exposure to cycling hypoxia but returns after reoxygenation.
MedLine Citation:
PMID:  22132723     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABSTRACT Purpose: Investigate the effect of cycling hypoxia on low dose hyper-radiosensitivity (HRS). Materials and methods: Human breast tumor T-47D cells were grown in a hypoxia workstation operated at 4% O(2) for 3-6 weeks and the pericellular oxygen concentration was recorded every 20 minutes. The presence of HRS in response to subsequent challenge irradiation was measured by clonogenic survival. Results: T-47D cells adapted to growing with 4% O(2) in the gas phase but showed no HRS. However, HRS was recovered after between 48 h and 2 weeks of reoxygenation at 20% O(2). Medium transferred from the hypoxic T-47D cells removed HRS in recipient cells grown in ambient air. Cells irradiated with X-rays showed a shallower HRS-"dip" and a lower d(c)-value (dose where the change from the hypersensitive to the induced repair response is 63% complete) compared to cells irradiated with (60)Coγ-rays. Conclusions: Cycling hypoxia transiently eliminates HRS in T-47D cells in vitro. This may partly explain the diverging results of in vivo studies of HRS. The effect of cycling hypoxia on HRS is comparable to our previous findings for T-47D cells receiving medium transfer from cells irradiated with 0.3 Gy at 0.3 Gy/h.
Authors:
Nina Jeppesen Edin; Dag Rune Olsen; Joe Alexander Sandvik; Eirik Malinen; Erik Olai Pettersen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-2
Journal Detail:
Title:  International journal of radiation biology     Volume:  -     ISSN:  1362-3095     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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