Document Detail

Low dose hyper-radiosensitivity is eliminated during exposure to cycling hypoxia but returns after reoxygenation.
MedLine Citation:
PMID:  22132723     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the effect of cycling hypoxia on low dose hyper-radiosensitivity (HRS).
MATERIALS AND METHODS: Human breast tumor T-47D cells were grown in a hypoxia workstation operated at 4% O(2) for 3-6 weeks and the pericellular oxygen concentration was recorded every 20 minutes. The presence of HRS in response to subsequent challenge irradiation was measured by clonogenic survival.
RESULTS: T-47D cells adapted to growing with 4% O(2) in the gas phase but showed no HRS. However, HRS was recovered after between 48 h and two weeks of reoxygenation at 20% O(2). Medium transferred from the hypoxic T-47D cells removed HRS in recipient cells grown in ambient air. Cells irradiated with X-rays showed a shallower HRS-'dip' and a lower d(c)-value (dose where the change from the hypersensitive to the induced repair response is 63% complete) compared to cells irradiated with (60)Co γ-rays.
CONCLUSIONS: Cycling hypoxia transiently eliminates HRS in T-47D cells in vitro. This may partly explain the diverging results of in vivo studies of HRS. The effect of cycling hypoxia on HRS is comparable to our previous findings for T-47D cells receiving medium transfer from cells irradiated with 0.3 Gy at 0.3 Gy/h.
Nina Jeppesen Edin; Dag Rune Olsen; Joe Alexander Sandvik; Eirik Malinen; Erik Olai Pettersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-06
Journal Detail:
Title:  International journal of radiation biology     Volume:  88     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-02     Completed Date:  2012-05-18     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  311-9     Citation Subset:  IM; S    
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MeSH Terms
Cell Count
Cell Hypoxia / physiology
Cell Line, Tumor
Dose-Response Relationship, Radiation
Oxygen / metabolism*
Radiation Tolerance / radiation effects*
Time Factors
Reg. No./Substance:

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