| Low dose cyclophosphamide rescues myocardial function from ischemia-reperfusion in rats. | |
| | |
MedLine Citation:
|
PMID: 18583145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: The effect of low dose of cyclophosphamide (CP) protecting cardiac function from ischemia-reperfusion injury was studied on rats. The premise is that CP inhibits immune and inflammatory process, thereby limits I/R injury and improves myocardial function. METHODS: Open chest rats were submitted to 30 min of ischemia followed by 3h, 12h or 24h of reperfusion. Rats were divided into sham group, I/R group and CP group, and each group included 3 time-point subgroups (3h, 12h and 24h; n=8 for each subgroup). A total of 750 mg/m(2) cyclophosphamide was intraperitoneally administrated in CP group and saline was given to I/R group. A polyethylene tube was inserted into the left ventricular cavity to detect left ventricular systolic pressure (LVSP) and +/-dp/dt(max). At the end of the experiment, hearts were harvested for histopathological assessment and infarct size determination. RESULTS: Compared with I/R group, rats treated with low dose CP showed a significant recovery in myocardial function with improved LVSP (88+/-11 vs 69+/-11 mmHg of 3h; 92+/-11 vs 64+/-14 mmHg of 12h; 90+/-11 vs 64+/-14 mmHg of 24h; p<0.01 respectively). The +/-dp/dt(max) also had the similar trends. The myocardial infarct size was reduced in CP group compared to that in I/R group; the infiltration of polymorph nuclear leukocytes (PMNs) in myocardium was decreased in CP group. The histopathological damage score was also attenuated. CONCLUSIONS: These findings suggest that low dose CP rescues cardiac function from ischemia-reperfusion injury by alleviating histopathological damage in rat myocardium. |
| | |
Authors:
|
Jianhua Zhu; Yuangang Qiu; Qiqi Wang; Yujuan Zhu; Shenjiang Hu; Liangrong Zheng; Lihong Wang; Yun Zhang |
Related Documents
:
|
18275975 - Co-application of ischemic preconditioning and postconditioning provides additive neuro... 15306165 - Attenuation of ischemia/reperfusion-induced intestinal injury by oleo-resin from copaif... 12553715 - Nadph-diaphorase activity in the spinal cord after ischemic injury and the effects of p... 12631355 - Preconditioning with tin-protoporphyrin ix attenuates ischemia/reperfusion injury in th... 9272695 - Increase in levels of total free fatty acids in rat brain regions following 3-nitroprop... 8819515 - In vivo depletion of free thiols does not account for nitroglycerin-induced tolerance: ... |
Publication Detail:
|
Type: Journal Article Date: 2008-06-26 |
Journal Detail:
|
Title: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery Volume: 34 ISSN: 1873-734X ISO Abbreviation: Eur J Cardiothorac Surg Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-08-18 Completed Date: 2009-10-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8804069 Medline TA: Eur J Cardiothorac Surg Country: Germany |
Other Details:
|
Languages: eng Pagination: 661-6 Citation Subset: IM |
Affiliation:
|
Department of Cardiology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cyclophosphamide / administration & dosage, therapeutic use* Disease Models, Animal Drug Administration Schedule Drug Evaluation, Preclinical / methods Immunosuppressive Agents / administration & dosage, therapeutic use* Male Myocardial Infarction / pathology, prevention & control Myocardial Reperfusion Injury / pathology, prevention & control* Neutrophil Infiltration / drug effects Rats Rats, Sprague-Dawley Ventricular Function, Left / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Immunosuppressive Agents; 50-18-0/Cyclophosphamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The early and late results of combined off-pump coronary artery bypass grafting and pulmonary resect...
Next Document: Polyunsaturated fatty acids as antioxidants.