| Low-dose lipopolysaccharide selectively sensitizes hypoxic ischemia-induced white matter injury in the immature brain. | |
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MedLine Citation:
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PMID: 20351655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Little is known about roles of inflammation and hypoxic ischemia (HI) in the generation of neuroinflammation and damage of blood-brain barrier (BBB) in the white matter (WM) that displays regional vulnerability in preterm infants. We investigated whether low-dose lipopolysaccharide (LPS) sensitizes HI-induced WM injury in postpartum (P) day 2 rat pups by selectively increasing neuroinflammation and BBB damage in the WM. Pups received LPS (0.05 mg/kg) (LPS + HI) or normal saline (NS + HI) followed by 90-min HI. LPS and NS group were the pups that had LPS or NS only. Myelin basic protein immunohistochemistry on P11 showed WM injury in LPS + HI group, but not in NS + HI, LPS, and NS groups. In contrast, no gray matter injury was found in the four groups. LPS + HI group also showed decreased number of oligodendrocytes in the WM 72-h postinsult. In the same brain region, increases of activated microglia, TNF-alpha expression, BBB leakage, and cleaved caspase-3 positive cells were much more prominent in LPS + HI group than in the other three groups 24-h postinsult. The oligodendrocytes were the major cells with cleaved caspase-3 expression. We concluded that low-dose LPS sensitized HI-induced WM injury in the immature brain by selectively up-regulating neuroinflammation and BBB damage in the WM. |
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Authors:
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Lan-Wan Wang; Ying-Chao Chang; Chang-Yi Lin; Jau-Shyong Hong; Chao-Ching Huang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pediatric research Volume: 68 ISSN: 1530-0447 ISO Abbreviation: Pediatr. Res. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-16 Completed Date: 2010-09-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
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Languages: eng Pagination: 41-7 Citation Subset: IM |
Affiliation:
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Institutes of Clinical Medicine, National Cheng Kung University College of Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Kaohsiung 833, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Apoptosis / drug effects Blood-Brain Barrier / drug effects, metabolism, pathology Brain* / drug effects, metabolism, pathology Female Humans Hypoxia-Ischemia, Brain* / metabolism, pathology Inflammation / metabolism, pathology Lipopolysaccharides / pharmacology* Nerve Fibers, Myelinated* / drug effects, pathology Oligodendroglia / cytology, drug effects, physiology Pregnancy Premature Birth Random Allocation Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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