| Low density lipoproteins interact with acidic fibroblast growth factor and modify its function. | |
| | |
MedLine Citation:
|
PMID: 12692004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Oxidized LDL (oxLDL) was shown to trigger the release of acidic fibroblast growth factor (FGF-1). Because these components are likely to be present simultaneously in the atherosclerotic milieu, we investigated whether oxLDL interacts with FGF-1 and whether this interaction affects FGF-1 functioning. METHODS AND RESULTS: Using molecular sieve and electrophoretic mobility shift assays, we found that FGF-1 forms a complex with oxLDL in vitro, in contrast to its low affinity for nonatherogenic, native LDL. The FGF-1/oxLDL complex had a dramatically decreased ability to bind heparin and was nonmitogenic on cultured smooth muscle cells. In human atherosclerotic lesions, the highest FGF-1 immunoreactivity was found in macrophages. With respect to oxLDL accumulation, 2 patterns were distinguished: (1) moderate, intracellular in matrix-rich regions containing viable cells and (2) massive, both cell-associated and extracellular oxLDL deposits in foam cell-rich regions with necrotic areas. The proliferating cell nuclear antigen readings for proliferating cells reflected that the mitogenic activity of FGF-1 was confined to the regions where oxLDL was strictly intracellular and was inhibited in the regions with extracellular oxLDL deposition. CONCLUSIONS: oxLDL, besides being a bulky component of the atherosclerotic lesion, possibly manifests its pathogenicity by complexing FGF-1 and inhibiting its growth-promoting function during atherogenesis. |
| | |
Authors:
|
Natalya Ananyeva; Alexey Tjurmin; Evgueni Saenko; Christian Haudenschild |
Related Documents
:
|
8468534 - Intracellular maturation of apolipoprotein[a] and assembly of lipoprotein[a] in primary... 10853684 - Direct and indirect effects of pulsatile shear stress on the smooth muscle cell. 7811744 - Evaluation of cultured hamster hepatocytes as an experimental model for the study of ve... 3156864 - An antiproliferative heparan sulfate species produced by postconfluent smooth muscle ce... 19604924 - Effects of pentylenetetrazol on resting membrane potential and on action potentials of ... 7273274 - Phorbol related esters have similar structure-activity relations in swiss 3t3 cells and... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2003-03-06 |
Journal Detail:
|
Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 23 ISSN: 1524-4636 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 2003 Apr |
Date Detail:
|
Created Date: 2003-04-14 Completed Date: 2004-01-15 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 601-7 Citation Subset: IM |
Affiliation:
|
Department of Experimental Pathology, American Red Cross, 15601 Crabbs Branch Way, Rockville, Md 20855, USA. ananyeva@usa.redcross.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Coronary Artery Disease
/
metabolism,
pathology Fibroblast Growth Factor 1 / metabolism, pharmacology*, secretion Foam Cells / metabolism Heparin / metabolism Humans Lipoproteins, LDL / pharmacology* Macromolecular Substances Macrophages / metabolism Peptide Fragments / pharmacology Protein Binding / drug effects Recombinant Proteins / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
HL 54246/HL/NHLBI NIH HHS; HL66101/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Lipoproteins, LDL; 0/Macromolecular Substances; 0/Peptide Fragments; 0/Recombinant Proteins; 0/fibroblast growth factor-1 (21-154), human; 0/oxidized low density lipoprotein; 104781-85-3/Fibroblast Growth Factor 1; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Systemic arterial expression of matrix metalloproteinases 2 and 9 in acute Kawasaki disease.
Next Document: Nebivolol prevents vascular NOS III uncoupling in experimental hyperlipidemia and inhibits NADPH oxi...