Document Detail

Low density lipoproteins from human ascites plasma. Characterization and degradation by sodium deoxycholate.
MedLine Citation:
PMID:  7390988     Owner:  NLM     Status:  MEDLINE    
The low density lipoproteins (LDL) in human ascites plasma were separated into two sublcasses by ultracentrifugation. The chemical compositions of the two LDLs were determined. On a weight percentage basis, LDL1 contained cholesterol ester, 20.8%; cholesterol, 19.3%; triacylglycerol, 19.7%; phospholipid, 22.1%; and protein, 10.8%. LDL2 contained cholesterol ester, 21.6%; cholesterol, 14.5%; triacylglycerol, 20.1%; phospholipid, 20.6%; and protein, 18.0%. The relative content of triacylglycerol and the ratio of unesterified cholesterol to esterified cholesterol in both LDLs were very high compared to corresponding human serum LDLs. Following the treatment of these LDLs with 575 mg of sodium deoxycholate per 10 mg of lipoprotein protein, a triacylglycerol-protein rich fraction was obtained by gel filtration in the presence of a micellar concentration of the detergent.
N Suzuki; S Kawashima; K Deguchi; N Ueta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  87     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1980 Apr 
Date Detail:
Created Date:  1980-09-23     Completed Date:  1980-09-23     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1253-6     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Apolipoproteins / analysis
Ascites / metabolism*
Cholesterol / analysis
Cholesterol Esters / analysis
Deoxycholic Acid
Lipoproteins, LDL* / isolation & purification
Phospholipids / analysis
Triglycerides / analysis
Reg. No./Substance:
0/Amino Acids; 0/Apolipoproteins; 0/Cholesterol Esters; 0/Lipoproteins, LDL; 0/Phospholipids; 0/Triglycerides; 57-88-5/Cholesterol; 83-44-3/Deoxycholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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