| Low density lipoproteins from human ascites plasma. Characterization and degradation by sodium deoxycholate. | |
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MedLine Citation:
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PMID: 7390988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The low density lipoproteins (LDL) in human ascites plasma were separated into two sublcasses by ultracentrifugation. The chemical compositions of the two LDLs were determined. On a weight percentage basis, LDL1 contained cholesterol ester, 20.8%; cholesterol, 19.3%; triacylglycerol, 19.7%; phospholipid, 22.1%; and protein, 10.8%. LDL2 contained cholesterol ester, 21.6%; cholesterol, 14.5%; triacylglycerol, 20.1%; phospholipid, 20.6%; and protein, 18.0%. The relative content of triacylglycerol and the ratio of unesterified cholesterol to esterified cholesterol in both LDLs were very high compared to corresponding human serum LDLs. Following the treatment of these LDLs with 575 mg of sodium deoxycholate per 10 mg of lipoprotein protein, a triacylglycerol-protein rich fraction was obtained by gel filtration in the presence of a micellar concentration of the detergent. |
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Authors:
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N Suzuki; S Kawashima; K Deguchi; N Ueta |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemistry Volume: 87 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1980 Apr |
Date Detail:
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Created Date: 1980-09-23 Completed Date: 1980-09-23 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1253-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Apolipoproteins / analysis Ascites / metabolism* Cholesterol / analysis Cholesterol Esters / analysis Deoxycholic Acid Humans Lipoproteins, LDL* / isolation & purification Phospholipids / analysis Triglycerides / analysis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Apolipoproteins; 0/Cholesterol Esters; 0/Lipoproteins, LDL; 0/Phospholipids; 0/Triglycerides; 57-88-5/Cholesterol; 83-44-3/Deoxycholic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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