| Low-density lipoprotein induced actin cytoskeleton reorganization in endothelial cells: mechanisms of action. | |
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MedLine Citation:
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PMID: 11577705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The inhibitory effects of the specific NADPH oxidase inhibitor, apocynin, and non-specific NADPH oxidase inhibitors, nordihydroguaiaretic acid (NDGA) and SKF525A, on the disruption of dense peripheral bands and formation of stress fibers in cultured human umbilical vein endothelial cells exposed to atherogenic low-density lipoprotein (LDL) levels has been investigated. Endothelial cells (EC) in vitro and in vivo exposed to high LDL-cholesterol levels have cytoskeletal remodeling with stress fiber formation and loss of dense peripheral bands. Cultured EC incubated with exogenously applied hydrogen peroxide (H2O2: 1 mM) have cytoskeletal structural changes much similar to those observed with high LDL exposure. Previous studies have 1) demonstrated that exposure to atherogenic LDL levels causes heightened EC H2O2 production, 2) identified the reactive oxygen species source, NADPH oxidase, in EC, and 3) shown that the specific NADPH oxidase inhibitor, apocynin, and non-specific NADPH oxidase inhibitors, NDGA and SKF525A, suppress H2O2 production increases in high LDL-perturbed EC. In the present study, the cytoskeletal structure of EC exposed to 330 mg/dl LDL-cholesterol, and incubated with or without apocynin, NDGA and SKF525A, was examined. Each of these compounds promoted the retention of dense peripheral bands and minimized stress fiber formation. These findings are consistent with NADPH oxidase and it's reactive oxygen species byproducts modulating the cytoskeleton reorganization observed in high LDL-induced EC perturbation. |
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Authors:
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J A Holland; R A Goss; R W O'Donnell; M M Chang; D K Johnson; L M Ziegler |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endothelium : journal of endothelial cell research Volume: 8 ISSN: 1062-3329 ISO Abbreviation: Endothelium Publication Date: 2001 |
Date Detail:
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Created Date: 2001-09-26 Completed Date: 2002-02-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9412590 Medline TA: Endothelium Country: Switzerland |
Other Details:
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Languages: eng Pagination: 117-35 Citation Subset: IM |
Affiliation:
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Department of Medicine, State University of New York Health Science Center Syracuse, 13210, USA. james.holland3@med.va.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetophenones
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pharmacology Actins / ultrastructure* Animals Aorta, Abdominal / ultrastructure Aorta, Thoracic / ultrastructure Arteriosclerosis / chemically induced, pathology Cells, Cultured Endothelium, Vascular / cytology, drug effects*, metabolism, ultrastructure* Enzyme Inhibitors / pharmacology Humans Lipoproteins, LDL / pharmacology* Male Microfilaments / drug effects, ultrastructure* Microscopy, Fluorescence NADPH Oxidase / antagonists & inhibitors Nordihydroguaiaretic Acid / pharmacology Perfusion Proadifen / pharmacology Rabbits Umbilical Veins / cytology |
| Grant Support | |
ID/Acronym/Agency:
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HL49573/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acetophenones; 0/Actins; 0/Enzyme Inhibitors; 0/Lipoproteins, LDL; 302-33-0/Proadifen; 498-02-2/acetovanillone; 500-38-9/Nordihydroguaiaretic Acid; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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