Document Detail

Low-density lipoprotein fraction from hen's egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane.
MedLine Citation:
PMID:  14613896     Owner:  NLM     Status:  MEDLINE    
For sperm preservation, semen is generally diluted with extender containing egg yolk (EY), but the mechanisms of sperm protection by EY are unclear. The major proteins of bull seminal plasma (BSP proteins: BSP-A1/A2, BSP-A3, and BSP-30-kDa) bind to sperm surface at ejaculation and stimulate cholesterol and phospholipid efflux from the sperm membrane. Since EY low-density lipoprotein fraction (LDF) interacts specifically with BSP proteins, it is proposed that the sequestration of BSP proteins in seminal plasma by EY-LDF represents the major mechanism of sperm protection by EY. In order to gain further insight into this mechanism, we investigated the effect of seminal plasma, EY, and EY-LDF on the binding of BSP proteins to sperm and the lipid efflux from the sperm membrane. As shown by immunodetection, radioimmunoassays, and lipid analysis, when semen was incubated undiluted or diluted with control extender (without EY or EY-LDF), BSP proteins bound to sperm in a time-dependent manner, and there is a continuous cholesterol and phospholipid efflux from the sperm membrane. In contrast, when semen was diluted with extender containing EY or EY-LDF, there was 50%-80% fewer BSP proteins associated with sperm and a significant amount of lipid added to sperm membrane during incubation. In addition, sperm function analysis showed that the presence of EY or EY-LDF in the extender preserved sperm motility. These results show that LDF is the constituent of EY that prevents binding of the BSP proteins to sperm and lipid efflux from the sperm membrane and is beneficial to sperm functions during sperm preservation.
Annick Bergeron; Marie-Hélène Crête; Yves Brindle; Puttaswamy Manjunath
Related Documents :
24962486 - Neurocysticercosis presenting as focal hydrocephalus.
3355566 - Aspirin prevents the nonenzymatic glycosylation and carbamylation of the human eye lens...
24487046 - Half-fluence versus half-dose photodynamic therapy in chronic central serous chorioreti...
21097546 - Eye movements in amyotrophic lateral sclerosis and its mimics: a review with illustrati...
16374046 - Retinal angiomatous proliferation: association with clinical and angiographic features.
11162756 - The usefulness of sleep and sleep deprivation as activating methods in electroencephalo...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-11-12
Journal Detail:
Title:  Biology of reproduction     Volume:  70     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-23     Completed Date:  2004-11-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  708-17     Citation Subset:  IM    
Departement of Medicine, University of Montreal, and Guy-Bernier Research Center, Maisonneuve-Rosemont Hospital, Montreal, Québec, Canada H1T 2M4.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Western
Cell Membrane / metabolism
Cholesterol / metabolism
Cold Temperature
Egg Yolk / metabolism*
Electrophoresis, Polyacrylamide Gel
Lipoproteins, LDL / metabolism*
Phosphatidylcholines / metabolism
Reproductive Techniques, Assisted / veterinary*
Semen / cytology,  metabolism
Semen Preservation
Seminal Vesicle Secretory Proteins / metabolism*
Spermatozoa / metabolism*
Reg. No./Substance:
0/BSP-30-kDa protein, Bos taurus; 0/Lipoproteins, LDL; 0/Phosphatidylcholines; 0/SVS8 protein, Bos taurus; 0/Seminal Vesicle Secretory Proteins; 0/seminal vesicle secretory protein 109, Bos taurus; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Retinoic acid inhibits rat XY gonad development by blocking mesonephric cell migration and decreasin...
Next Document:  Involvement of mitogen-activated protein kinase cascade during oocyte maturation and fertilization i...