Document Detail


Low density lipoprotein-containing circulating immune complexes and coronary atherosclerosis.
MedLine Citation:
PMID:  2369935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blood serum of most patients with coronary heart disease (CHD) caused a 2- to 5-fold increase in the lipid content of smooth muscle cells cultured from unaffected human aortic intima, i.e., possessed an atherogenic potential manifested in culture. Treatment of the CHD patients' serum with 2.5% polyethylene glycol 6000 removed the circulating immune complexes. The serum subjected to this treatment lost its atherogenic properties, i.e., failed to increase the content of lipids in cultured cells. Incubation of smooth muscle cells derived from human aortic intima with circulating immune complexes isolated from an atherogenic patient's serum caused a 1.5- to 3-fold rise in the intracellular cholesterol. Circulating immune complexes contained apolipoprotein B (apo B), but not apolipoproteins A1 and E. The apo B content strongly correlated with the total cholesterol content. The cholesterol/apo B ratio of the complexes was characteristic of low density lipoproteins (LDL), but not of very low density lipoproteins or intermediate density lipoproteins. The composition of the main lipid classes in these complexes was similar to that in LDL. Blood sera of most (90%) CHD patients was characterized by a high cholesterol and apolipoprotein B content in circulating immune complexes. The ability of these sera to induce lipid accumulation in cultured cells directly correlated with the cholesterol and apolipoprotein B level of circulating immune complexes (r = 0.91). These findings suggest that the atherogenic potential of CHD patients' blood serum is due to LDL-containing immune complexes.
Authors:
V V Tertov; A N Orekhov; A G Kacharava; I A Sobenin; N V Perova; V N Smirnov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  52     ISSN:  0014-4800     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-21     Completed Date:  1990-08-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  300-8     Citation Subset:  IM    
Affiliation:
USSR Cardiology Research Center, Moscow.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigen-Antibody Complex*
Apolipoproteins B / metabolism
Arteriosclerosis / etiology*
Cells, Cultured
Coronary Disease / blood*
Female
Humans
Lipoproteins, LDL / blood*,  immunology
Male
Middle Aged
Muscle, Smooth, Vascular / metabolism
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Apolipoproteins B; 0/Lipoproteins, LDL

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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