| Low-density lipoprotein (LDL) oxidizability before and after LDL apheresis. | |
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MedLine Citation:
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PMID: 10421230 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidation of low-density lipoprotein (LDL) plays a major role in the development of atherosclerosis. Hypercholesterolemia has been associated with enhanced in vitro oxidation of LDL, and lipid-lowering therapy reduces LDL oxidizability. In the present study, we investigated whether LDL apheresis performed with different techniques affects in vitro diene formation (lag phase) and modification of apolipoprotein B-100 (apoB). Baseline and posttreatment diene formation was correlated with the baseline pattern of plasma total fatty acids. We then performed a computer-simulation study to test the hypothesis that LDL apheresis-induced changes in LDL oxidizability are related to changes in the mass ratio between freshly produced and older LDL. In 19 patients aged 49+/-7 years with heterozygous familial hypercholesterolemia (FH) regularly treated with either immunoadsorption, heparin-induced LDL precipitation (HELP), or dextran sulfate (DS) adsorption, we determined lipoprotein levels, the lag phase, apoB modification, and the fatty acid pattern in plasma samples drawn at the onset and termination of one LDL apheresis. LDL apheresis significantly decreased total cholesterol, high-density lipoprotein (HDL) cholesterol, LDL cholesterol, and triglycerides by 50.4%, 14.9%, 62.6%, and 33.6%, respectively. The lag phase increased by a significant mean of 9.8%; the charge of apoB was not altered. The lag phase before treatment positively correlated with the baseline concentration of plasma total palmitic, myristic, and oleic acid. The increase in the lag phase during treatment correlated with a high pretreatment concentration of lauric, linoleic, and docosahexanoic acid. The simulation study indicates that a temporary imbalance between two LDL compartments, one representing freshly secreted LDL and the other representing older LDL, could explain the observed increase in the lag phase after LDL apheresis. In conclusion, in patients with heterozygous FH, LDL apheresis performed with different techniques decreases the susceptibility of LDL to oxidation. This decrease may be related to a temporary mass imbalance between freshly produced and older LDL particles. Furthermore, the baseline fatty acid pattern influences pretreatment and posttreatment susceptibility to oxidation. |
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Authors:
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M G Donner; K G Parhofer; W O Richter; P Schwandt |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 48 ISSN: 0026-0495 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-08-03 Completed Date: 1999-08-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 881-6 Citation Subset: IM |
Affiliation:
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Medical Department II, University of Munich, Klinikum Grosshadern, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apolipoprotein B-100 Apolipoproteins B / blood Blood Component Removal* Computer Simulation Dextran Sulfate / therapeutic use Fatty Acids / blood Female Heparin / therapeutic use Humans Hyperlipoproteinemia Type II / blood, drug therapy, therapy Immunosorbents / therapeutic use Lipoproteins / blood Lipoproteins, LDL / metabolism* Male Middle Aged Oxidation-Reduction |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein B-100; 0/Apolipoproteins B; 0/Fatty Acids; 0/Immunosorbents; 0/Lipoproteins; 0/Lipoproteins, LDL; 9005-49-6/Heparin; 9042-14-2/Dextran Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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