Document Detail

Low cost CD4 enumeration using generic monoclonal antibody reagents and a two-color user-defined MultiSET protocol.
MedLine Citation:
PMID:  16906545     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The standard three-tube, three-color flow cytometric method utilizing the TriTEST reagents in conjunction with the MultiSET software commonly used in most laboratories in Thailand for CD4 enumeration is expensive and thus unavailable to most HIV-infected patients. A more affordable method, i.e., the PanLeucogating protocol using only two monoclonal antibody reagents, has been described but requires the use of the CellQUEST software that does not have automatic gating and reporting facilities. We describe a simple protocol that utilizes a two-color user-defined protocol with the automated MultiSET software for the acquisition, analysis, and reporting of CD4 results. METHODS: A two-color user-defined protocol was set up following instructions in the Becton Dickinson Biosciences MultiSET manual, adhering strictly to the information regarding the Gate and Attractor Hierarchy for analyzing various reagent combinations. This simple two-color user-defined MultiSET software was evaluated using generic monoclonal reagents in comparison with the standard TriTEST/MultiSET protocol. RESULTS: The two-color user-defined MultiSET software is easy to use. It requires only modification of the original MultiSET program and the results obtained are comparable with those derived from the standard TriTEST/MultiSET protocol. CONCLUSION: The use of this easy and reliable two-color user-defined MultiSET protocol represents an affordable alternative to CD4 testing in resource-poor settings.
Kovit Pattanapanyasat; Hla Shain; Varipin Prasertsilpa; Egarit Noulsri; Surada Lerdwana; Achara Eksaengsri
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry. Part B, Clinical cytometry     Volume:  70     ISSN:  1552-4949     ISO Abbreviation:  Cytometry B Clin Cytom     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-21     Completed Date:  2006-11-14     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101235690     Medline TA:  Cytometry B Clin Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-60     Citation Subset:  IM    
Copyright Information:
Copyright 2006 International Society for Analytical Cytology.
Center of Excellence for Flow Cytometry, Office for Research and Development, Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use*
Antigens, CD45 / metabolism
CD4 Lymphocyte Count / methods*
Flow Cytometry
HIV Infections / diagnosis*,  immunology*
Image Processing, Computer-Assisted
Sensitivity and Specificity
Reg. No./Substance:
0/Antibodies, Monoclonal; EC, CD45

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