| Low concentrations of a non-hydrolysable tetra-S-glycosylated porphyrin and low light induces apoptosis in human breast cancer cells via stress of the endoplasmic reticulum. | |
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MedLine Citation:
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PMID: 18958330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A water-soluble tetra-S-glycosylated porphyrin (P-Glu(4)) is absorbed by MDA-MB-231 human breast cancer cells whereupon irradiation with visible light causes necrosis or apoptosis depending on the concentration of the porphyrin and the power of the light. With the same amount of light irradiation power (9.4 W m(-2)), at 10-20 microM concentrations necrosis is predominantly observed, while at <10 microM concentrations, apoptosis is the principal cause of cell death. Of the various possible pathways for the induction of apoptosis, experiments demonstrate that calcium is released from the endoplasmic reticulum, cytochrome c is liberated from the mitochondria to the cytosol, pro-caspase-3 is activated, poly-(ADP-ribose) polymerase is cleaved, and the chromatin is condensed subsequent to photodynamic treatment of these cells. Confocal microscopy indicates a substantial portion of the P-Glu(4) is located in the endoplasmic reticulum at <10 microM. These data indicate that the photodynamic treatment of MDA-MB-231 cells using low concentrations of the P-Glu(4) porphyrin and low light induces apoptosis mostly initiated from stress produced to the endoplasmic reticulum. |
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Authors:
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Sebastian Thompson; Xin Chen; Li Hui; Alfredo Toschi; David A Foster; Charles Michael Drain |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-08-18 |
Journal Detail:
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Title: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Volume: 7 ISSN: 1474-905X ISO Abbreviation: Photochem. Photobiol. Sci. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-29 Completed Date: 2009-03-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101124451 Medline TA: Photochem Photobiol Sci Country: England |
Other Details:
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Languages: eng Pagination: 1415-21 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Hunter College and Graduate Center of the City University of New York, New York, NY 10065, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects*, radiation effects* Breast Neoplasms / pathology* Calcium / metabolism Caspases / metabolism Cattle Cell Line, Tumor Cytochromes c / metabolism Endoplasmic Reticulum / drug effects, metabolism*, radiation effects Enzyme Activation / drug effects Glycosylation Hydrolysis Indoles Light* Microscopy, Confocal Microscopy, Fluorescence Mitochondria / metabolism Poly(ADP-ribose) Polymerases / metabolism Porphyrins / chemistry*, pharmacology* Staining and Labeling Stress, Physiological / drug effects, radiation effects |
| Grant Support | |
ID/Acronym/Agency:
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CA46677/CA/NCI NIH HHS; GM60654/GM/NIGMS NIH HHS; RR-03037/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 0/Porphyrins; 47165-04-8/DAPI; 7440-70-2/Calcium; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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