| Low complex I content explains the low hydrogen peroxide production rate of heart mitochondria from the long-lived pigeon, Columba livia. | |
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MedLine Citation:
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PMID: 19968628 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Across a range of vertebrate species, it is known that there is a negative association between maximum lifespan and mitochondrial hydrogen peroxide production. In this report, we investigate the underlying biochemical basis of the low hydrogen peroxide production rate of heart mitochondria from a long-lived species (pigeon) compared with a short-lived species with similar body mass (rat). The difference in hydrogen peroxide efflux rate was not explained by differences in either superoxide dismutase activity or hydrogen peroxide removal capacity. During succinate oxidation, the difference in hydrogen peroxide production rate between the species was localized to the DeltapH-sensitive superoxide producing site within complex I. Mitochondrial DeltapH was significantly lower in pigeon mitochondria compared with rat, but this difference in DeltapH was not great enough to explain the lower hydrogen peroxide production rate. As judged by mitochondrial flavin mononucleotide content and blue native polyacrylamide gel electrophoresis, pigeon mitochondria contained less complex I than rat mitochondria. Recalculation revealed that the rates of hydrogen peroxide production per molecule of complex I were the same in rat and pigeon. We conclude that mitochondria from the long-lived pigeon display low rates of hydrogen peroxide production because they have low levels of complex I. |
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Authors:
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Adrian J Lambert; Julie A Buckingham; Helen M Boysen; Martin D Brand |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-25 |
Journal Detail:
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Title: Aging cell Volume: 9 ISSN: 1474-9726 ISO Abbreviation: Aging Cell Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-18 Completed Date: 2010-02-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101130839 Medline TA: Aging Cell Country: England |
Other Details:
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Languages: eng Pagination: 78-91 Citation Subset: IM |
Affiliation:
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Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 0XY, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging* Animals Antioxidants / metabolism Avian Proteins / metabolism* Columbidae / metabolism* Electron Transport Complex I / metabolism* Female Hydrogen Peroxide / metabolism* Male Mitochondria, Heart / metabolism* Oxidation-Reduction Rats Rats, Wistar |
| Grant Support | |
ID/Acronym/Agency:
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066750/B/01/Z//Wellcome Trust; //Medical Research Council |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Avian Proteins; 7722-84-1/Hydrogen Peroxide; EC 1.6.5.3/Electron Transport Complex I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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