Document Detail


Low clonogenic potential of circulating angiogenic cells is associated with lower density of capillaries in skeletal muscle in patients with impaired glucose tolerance.
MedLine Citation:
PMID:  23390082     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Reduced density of capillaries in skeletal muscle can limit insulin, glucose, and oxygen supply to the muscle, thereby contributing to worsening metabolism in older adults. The lower skeletal muscle capillarization in impaired glucose tolerance (IGT) may partially be due to circulating angiogenic cell dysfunction. Circulating angiogenic cells maintain the vasculature and promote angiogenesis, but circulating angiogenic cell number and function may be reduced in IGT. The goal of this study was to determine whether the clonogenic potential of circulating angiogenic cells is lower in IGT compared with normal-glucose-tolerant (NGT) controls and is associated with skeletal muscle capillarization.
METHODS: Glucose tolerance, endothelial cell colony-forming unit (CFU-EC) number, and vastus lateralis capillary density were measured in sedentary, older (62 ± 1 years, mean ± SEM) men and women with NGT (n = 16) and IGT (n = 12).
RESULTS: Adults with IGT had 43% lower CFU-EC number (11.4 ± 2.3 versus 20.1 ± 2.0 colonies, p < 0.01) and 12% lower capillary density (291 ± 11 versus 330 ± 9 capillaries/mm², p < 0.01) compared with those with NGT. In regression analyses, CFU-EC number inversely correlated with 120-min postprandial glucose in all subjects (r = -0.47, p < 0.05), and capillary density was directly associated with CFU-EC number (r = 0.53, p < 0.05).
CONCLUSIONS: We conclude that the clonogenic potential of circulating angiogenic cells is lower in sedentary older adults with IGT and is associated with lower skeletal muscle capillarization. Low circulating angiogenic cell clonogenic potential in IGT suggests a state of impaired angiogenesis occurring prior to overt type 2 diabetes that may mediate early microvascular changes in the development and progression of IGT to type 2 diabetes.
Authors:
Steven J Prior; Alice S Ryan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Diabetes/metabolism research and reviews     Volume:  29     ISSN:  1520-7560     ISO Abbreviation:  Diabetes Metab. Res. Rev.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-07     Completed Date:  2013-12-05     Revised Date:  2014-07-29    
Medline Journal Info:
Nlm Unique ID:  100883450     Medline TA:  Diabetes Metab Res Rev     Country:  England    
Other Details:
Languages:  eng     Pagination:  319-25     Citation Subset:  IM    
Copyright Information:
Published 2013. This article is a U.S. Government work and is in the public domain in the USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Diabetes Mellitus, Type 2 / metabolism,  pathology*
Female
Glucose Intolerance / metabolism,  pathology*
Glucose Tolerance Test
Humans
Male
Middle Aged
Muscle, Skeletal / blood supply*,  metabolism,  pathology
Neovascularization, Pathologic / blood*
Oxygen Consumption
Stem Cells
Grant Support
ID/Acronym/Agency:
K23 AG040775/AG/NIA NIH HHS; P30 AG028747/AG/NIA NIH HHS; P30 DK079637/DK/NIDDK NIH HHS; P30-AG028747/AG/NIA NIH HHS
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