Document Detail


A low mortality model of chronic pulmonary hypertension in sheep.
MedLine Citation:
PMID:  21529838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Pulmonary hypertension and right ventricular failure are major contributors to morbidity and mortality in chronic lung disease. Therefore, large animal models of pulmonary hypertension and right ventricular hypertrophy are needed to study underlying disease mechanisms and test new treatment modalities. The objective of this study was to create a low-mortality model of chronic pulmonary hypertension and right ventricular hypertrophy in sheep.
METHODS: The vena cavae of nine sheep weighing 62 ± 2 (SEM) kg were injected with 0.375 g of dextran beads (sephadex) every day for 60 d. Pulmonary hemodynamics were assessed via pulmonary artery catheterization prior to the first injection and again on d 14, 28, 35, 42, 49, and 56. At the end of the experiment, the heart was removed, dissected, and weighed to determine the ratio of right ventricular mass to left ventricle plus septal mass (RV:LV+S).
RESULTS: All sheep survived to 60 d. The average pulmonary artery pressure rose from 17 ± 1 mmHg at baseline to 35 ± 3 mmHg on d 56 with no significant change in cardiac output (8.7 ± 0.7 to 9.8 ± 0.7 L/min, P = 0.89). The RV:LV+S was significantly higher (0.42 ± 0.01, P < 0.001) than a historic group of untreated normal animals (0.35 ± 0.01, n = 13).
CONCLUSION: This study provides a low-mortality large animal model of moderate chronic pulmonary hypertension and right ventricular hypertrophy.
Authors:
Joshua R Pohlmann; Begum Akay; Daniele Camboni; Kelly L Koch; Benjamin M Mervak; Keith E Cook
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-09
Journal Detail:
Title:  The Journal of surgical research     Volume:  175     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-30     Completed Date:  2012-06-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chronic Disease
Disease Models, Animal*
Hypertension, Pulmonary / mortality*,  physiopathology
Hypertrophy, Right Ventricular / mortality*
Sheep
Grant Support
ID/Acronym/Agency:
R01 HL089043/HL/NHLBI NIH HHS; R01 HL089043-01A2/HL/NHLBI NIH HHS; R01 HL089043-04/HL/NHLBI NIH HHS; R01-HL089043/HL/NHLBI NIH HHS
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