| A low molecular weight folate receptor targeted contrast agent for magnetic resonance tumor imaging. | |
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MedLine Citation:
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PMID: 20809208 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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PURPOSE: This study aims to develop a low molecular weight folate receptor (FR) contrast agent for MR tumor imaging. PROCEDURES: Gadolinium-tetraazacyclododecane tetraacetic acid (Gd.DOTA) was conjugated to folic acid to create Gd.DOTA.Folate. The efficacy of Gd.DOTA.Folate to bind FR was evaluated in vitro by inductively coupled mass spectrometry (ICP-MS) and in vivo by magnetic resonance imaging (MRI) tumor enhancement over 14 h, utilizing an overexpressing α-FR cell line (IGROV-1), compared to an α-FR-negative cell line (OVCAR-3). Gd.DOTA.Folate localization ex vivo was verified by laser ablation ICP-MS. RESULTS: ICP-MS confirmed Gd.DOTA.Folate uptake by IGROV-1 cells and competitive binding with free folic acid inhibited binding. IGROV-1 tumors showed an increase in R (1) at 2 h, which increased significantly over 14 h post-Gd.DOTA.Folate with clear enhancement on MR images. This was not observed in controls. CONCLUSION: These data support the use of FR-targeted small molecular weight MRI contrast agents for tumor imaging in vivo. |
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Authors:
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Tammy L Kalber; Nazila Kamaly; Po-Wah So; John A Pugh; Josephine Bunch; Cameron W McLeod; Michael R Jorgensen; Andrew D Miller; Jimmy D Bell |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging Volume: 13 ISSN: 1860-2002 ISO Abbreviation: Mol Imaging Biol Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125610 Medline TA: Mol Imaging Biol Country: United States |
Other Details:
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Languages: eng Pagination: 653-62 Citation Subset: IM |
Affiliation:
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Metabolic and Molecular Imaging Group, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, London, W12 0NN, UK, tammy.kalber@csc.mrc.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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