Document Detail


Low-level laser therapy decreases renal interstitial fibrosis.
MedLine Citation:
PMID:  23134313     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: the purpose of this study was to investigate the effect of low-level laser therapy (LLLT) on chronic kidney disease (CKD) in a model of unilateral ureteral obstruction (UUO).
BACKGROUND DATA: Regardless of the etiology, CKD involves progressive widespread tissue fibrosis, tubular atrophy, and loss of kidney function. This process also occurs in kidney allograft. At present, effective therapies for this condition are lacking. We investigated the effects of LLLT on the interstitial fibrosis that occurs after experimental UUO in rats.
METHODS: The occluded kidney of half of the 32 Wistar rats that underwent UUO received a single intraoperative dose of LLLT (AlGaAs laser, 780 nm, 22.5 J/cm(2), 30 mW, 0.75 W/cm(2), 30 sec on each of nine points). After 14 days, renal fibrosis was assessed by Sirius red staining under polarized light. Immunohistochemical analyses quantitated the renal tissue cells that expressed fibroblast (FSP-1) and myofibroblast (α-SMA) markers. Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to determine the mRNA expression of interleukin (IL)-6, monocyte chemotactic protein-1 (MCP-1), transforming growth factor (TGF)-β1 and Smad3.
RESULTS: The UUO and LLLT animals had less fibrosis than the UUO animals, as well having decreased expression inflammatory and pro-fibrotic markers.
CONCLUSIONS: For the first time, we showed that LLLT had a protective effect regarding renal interstitial fibrosis. It is conceivable that by attenuating inflammation, LLLT can prevent tubular activation and transdifferentiation, which are the two processes that mainly drive the renal fibrosis of the UUO model.
Authors:
Fabiana Aparecida Mayrink Oliveira; Ana Carolina Meneghin Moraes; Amanda Povoa Paiva; Vânia Schinzel; Matheus Correa-Costa; Patricia Semedo; Angêla Castoldi; Marcos Antonio Cenedeze; Roberto Sotto-Maior Fortes Oliveira; Marcus Gomes Bastos; Niels Olsen Saraiva Câmara; Helady Sanders-Pinheiro
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-07
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  30     ISSN:  1557-8550     ISO Abbreviation:  Photomed Laser Surg     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-11-22     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  705-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy, Needle
Disease Models, Animal
Immunohistochemistry
Kidney Diseases / pathology,  radiotherapy
Laser Therapy, Low-Level / methods*
Male
Nephritis, Interstitial / pathology*,  radiotherapy*
Rats
Rats, Wistar
Sensitivity and Specificity
Treatment Outcome
Comments/Corrections
Comment In:
Photomed Laser Surg. 2013 Oct;31(10):507-8   [PMID:  24102169 ]
Photomed Laser Surg. 2013 Oct;31(10):505-6   [PMID:  23945023 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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