| Low CXCR4 membrane expression on CD34(+) cells characterizes cells mobilized to blood. | |
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MedLine Citation:
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PMID: 16247418 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Summary:In this work, association between the presence and membrane density of CXCR4 and the effectiveness of mobilization was studied. Ninety G-CSF mobilized PBPC and 28 native BM (nBM) preparations obtained from healthy individuals for transplantation and BM obtained after G-CSF mobilized PBPC collection in 10 donors were investigated. Positivity for CD34, HLA-DR and CXCR4 were analysed in the three colour fluorescence. The cellular profile of PBPC differed from nBM preparations with respect to lower: (i) proportion of CD34(+) cells (0.64%+/-0.04 vs 0.92+/-0.07) and CD34(+)CXCR4(+) cells (0.30%+/-0.02 vs 0.61+/-0.07); (ii) contribution of CXCR4(+) cells to CD34(+) cells (52.2%+/-2.5 vs 62.2%+/-4.2); (iii) CXCR4 epitope density in CD34(+) cells (48.9+/-5.5 vs 94.7+/-10.4). PBPC yield for CD34(+) cells was correlated with the content of CD34(+) cells lacking CXCR4 in the leukapheresis product (R=0.38). In contrast, nBM harvested for transplantation was poor in CD34(+) cells if these cells were frequently CXCR4- (R=-0.49). The present study shows that CD34(+) cells mobilized to blood were characterized with a low proportion of CXCR4 and this associated with CD34(+) cell content in PBPC. |
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Authors:
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D Dlubek; D Drabczak-Skrzypek; A Lange |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bone marrow transplantation Volume: 37 ISSN: 0268-3369 ISO Abbreviation: Bone Marrow Transplant. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2005-12-16 Completed Date: 2006-04-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8702459 Medline TA: Bone Marrow Transplant Country: England |
Other Details:
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Languages: eng Pagination: 19-23 Citation Subset: IM |
Affiliation:
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L. Hirszfeld Institute of Immunology and Experimental Therapy, Lower Silesian Centre for Cellular Transplantation and National Bone Marrow Donor Registry, Wroclaw, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD34
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biosynthesis* Bone Marrow Cells / cytology, metabolism* Flow Cytometry Gene Expression Regulation / drug effects, physiology* Granulocyte Colony-Stimulating Factor / administration & dosage Hematopoietic Stem Cell Mobilization Humans Leukapheresis Leukocytes / cytology, metabolism* Receptors, CXCR4 / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34; 0/Receptors, CXCR4; 143011-72-7/Granulocyte Colony-Stimulating Factor |
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