| Lovastatin arrests CHO cells between the origin decision point and the restriction point. | |
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MedLine Citation:
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PMID: 11068042 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Asynchronously growing Chinese hamster ovary (CHO) cells treated with the pro-drug, beta-lactone ring form of lovastatin were arrested in G(1)-phase. Subsequent removal of lovastatin resulted in the synchronous entry of cells into S-phase regardless of the presence of mevalonic acid. Lovastatin-arrested cells contained hypophosphorylated retinoblastoma protein (Rb) and required serum mitogens to enter S-phase after lovastatin removal, indicating that cell-cycle arrest is prior to the restriction point (R-point). However, in contrast to quiescent cells, intact nuclei prepared from lovastatin-arrested cells were competent for DNA replication when introduced into Xenopus egg extracts. Initiation of replication by Xenopus egg cytosol took place specifically within the dihydrofolate reductase (DHFR) origin locus, demonstrating that cells were arrested after the origin decision point (ODP). We conclude that the beta-lactone ring form of lovastatin is an effective reagent with which to synchronize CHO cells between the ODP and R-point, without resulting in the withdrawal of cells from the cell-cycle into a quiescent state. |
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Authors:
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J R Wu; D M Gilbert |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 484 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-17 Completed Date: 2000-12-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 108-12 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Shanghai Research Center of Life Science, Shanghai Institutes for Biological Sciences, Chineses Academy of Sciences, Shanghai, Japam. wujr@sunm.shcnc.ac.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / pharmacology CHO Cells Cricetinae DNA Replication / drug effects* G1 Phase / drug effects* Lovastatin / pharmacology* Prodrugs / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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GM57233/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Prodrugs; 75330-75-5/Lovastatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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