Document Detail

Loss of simian virus 40 DNA-RNA hybrids from nitrocellulose membranes; implications for the study of virus--host DNA interactions.
MedLine Citation:
PMID:  4340749     Owner:  NLM     Status:  MEDLINE    
Complete hybrids of simian virus 40 (SV40)DNA and its complementary RNA (cRNA) are not retained on nitrocellulose membranes. At saturating cRNA concentrations, retention of the hybrids indicates incomplete homology between DNA and RNA, probably due to incorporation of host DNA in the viral DNA; this effect is most pronounced when DNA is produced in cells infected at high multiplicity. Hybrids between DNA of Chinese hamster cells transformed by SV40 and cRNA are retained if the DNA fragments are long, but they are lost if the DNA is sheared to less than the length of an SV40 DNA molecule. Hence, in cells examined with about six SV40 genomes per cell, each genome is individually integrated. The results may explain previous discrepancies in the estimation of the number of viral genomes in transformed cells.
M Haas; M Vogt; R Dulbecco
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Aug 
Date Detail:
Created Date:  1972-10-05     Completed Date:  1972-10-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2160-4     Citation Subset:  IM    
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MeSH Terms
Cell Line
Centrifugation, Density Gradient
DNA, Bacterial
DNA, Viral* / isolation & purification
Escherichia coli
Membranes, Artificial
Molecular Weight
Nucleic Acid Hybridization*
Phosphorus Isotopes
RNA, Viral* / biosynthesis
Simian virus 40*
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Viral; 0/Membranes, Artificial; 0/Phosphorus Isotopes; 0/RNA, Viral; 10028-17-8/Tritium; 9004-34-6/Cellulose; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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